Cloned (Comment) | Organism |
---|---|
gene rpiB, expression in Escherichia coli strain ER2566 | Acetivibrio thermocellus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant RpiB, sitting drop vapour diffusion method, 0.001 ml of 7 mg/ml protein in mM Tris-HCl buffer pH 7.5 is mixed with 0.001 ml of reservoir solution, containing 0.05 M Tris, pH 7.0, 10% PEG 8000, 0.15 M MgCl2 and 0.2 M KCl, and equilibrated against 1 ml of reservoir solution, 3 weeks, flash-cooling in liquid nitrogen with a cryoprotectant solution containing 65 mM Tris pH 7.0, 13% PEG 8000, 195 mM MgCl2, 260 mM KCl and 20% glycerol, X-ray diffraction structure determination and analysis at 1.9 A resolution, modeling | Acetivibrio thermocellus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
about, native PAGE, recombinant enzyme | Acetivibrio thermocellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-allose | Acetivibrio thermocellus | - |
D-psicose | - |
r | |
D-ribose | Acetivibrio thermocellus | - |
D-ribulose | - |
r | |
D-ribose 5-phosphate | Acetivibrio thermocellus | preferred substrate | D-ribulose 5-phosphate | - |
r | |
D-talose | Acetivibrio thermocellus | - |
D-tagatose | - |
r | |
L-allose | Acetivibrio thermocellus | - |
L-psicose | - |
r | |
L-ribose | Acetivibrio thermocellus | - |
L-ribulose | - |
r | |
L-talose | Acetivibrio thermocellus | preferred substrate | L-tagatose | - |
r | |
additional information | Acetivibrio thermocellus | CTRPI has a narrow substrate specificity for rare sugars and reversibly converts aldose substrates containing hydroxyl groups oriented in the same direction to one of the corresponding ketoses. CTRPI prefers aldose substrates such as L-talose, D-ribose and D-allose | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
RpiB; gene rpiB | - |
Purification (Comment) | Organism |
---|---|
recombinant RpiB from Escherichia coli strain ER2566 by His affinity chromatography | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-allose | - |
Acetivibrio thermocellus | D-psicose | - |
r | |
D-ribose | - |
Acetivibrio thermocellus | D-ribulose | - |
r | |
D-ribose 5-phosphate | preferred substrate | Acetivibrio thermocellus | D-ribulose 5-phosphate | - |
r | |
D-talose | - |
Acetivibrio thermocellus | D-tagatose | - |
r | |
L-allose | - |
Acetivibrio thermocellus | L-psicose | - |
r | |
L-ribose | - |
Acetivibrio thermocellus | L-ribulose | - |
r | |
L-talose | preferred substrate | Acetivibrio thermocellus | L-tagatose | - |
r | |
additional information | CTRPI has a narrow substrate specificity for rare sugars and reversibly converts aldose substrates containing hydroxyl groups oriented in the same direction to one of the corresponding ketoses. CTRPI prefers aldose substrates such as L-talose, D-ribose and D-allose | Acetivibrio thermocellus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 x 17500, about, recombinant enzyme, RpiB has an active-site interface between the dimer structures | Acetivibrio thermocellus |
Synonyms | Comment | Organism |
---|---|---|
CTRPI | - |
Acetivibrio thermocellus |
D-ribose-5-phosphate isomerase | - |
Acetivibrio thermocellus |
RpiB | - |
Acetivibrio thermocellus |
General Information | Comment | Organism |
---|---|---|
metabolism | Rpi is involved in D-allose metabolism by converting D-ribose-5-phosphate to D-ribulose-5-phosphate and vice versa in a branch of the pentose phosphate pathway | Acetivibrio thermocellus |