Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Top10 cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
in complex with 5-deoxy-5-phospho-D-ribonohydroxamate, hanging drop vapour diffusion method, with 15% PEG 8000, 0.1 M MES buffer, pH 6, 5% PEG 1000, and 0.2 M Li2SO4, or in complex with D-ribose 5-phosphate, sitting drop vapour diffusion method, with 20% PEG 3K, 0.1 M Tris, pH 7, and 0.2 M Ca acetate | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-deoxy-5-phospho-D-ribonohydroxamate | - |
Mycobacterium tuberculosis | |
D-Allose 6-phosphate | - |
Mycobacterium tuberculosis | |
D-allulose 6-phosphate | - |
Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
D-ribose 5-phosphate | in 50 mM Tris-HCl, pH 7.5, at 37°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Mycobacterium tuberculosis | P9WKD7 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WKD7 | - |
- |
Purification (Comment) | Organism |
---|---|
gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribose 5-phosphate | - |
Escherichia coli | D-ribulose 5-phosphate | - |
? | |
D-ribose 5-phosphate | - |
Mycobacterium tuberculosis | D-ribulose 5-phosphate | - |
? | |
D-ribose 5-phosphate | - |
Mycobacterium tuberculosis H37Rv | D-ribulose 5-phosphate | - |
? | |
additional information | no activity with D-allose 6-phosphate | Mycobacterium tuberculosis | ? | - |
? | |
additional information | no activity with D-allose 6-phosphate | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-ribose-5-phosphate isomerase A | - |
Escherichia coli |
D-ribose-5-phosphate isomerase B | - |
Mycobacterium tuberculosis |
RpiA | - |
Escherichia coli |
RpiB | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
47 | - |
D-ribose 5-phosphate | in 50 mM Tris-HCl, pH 7.5, at 37°C | Mycobacterium tuberculosis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
2 | - |
in 50 mM Tris-HCl, pH 7.5, at 37°C | Mycobacterium tuberculosis | D-Allose 6-phosphate | |
6.3 | - |
in 50 mM Tris-HCl, pH 7.5, at 37°C | Mycobacterium tuberculosis | D-allulose 6-phosphate |