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Literature summary for 5.3.1.6 extracted from
- Ribose 5-phosphate isomerase type B from Trypanosoma cruzi: kinetic properties and site-directed mutagenesis reveal information about the reaction mechanism (2007), Biochem. J., 401, 279-285.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Trypanosoma cruzi |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C69A | no catalytic activity | Trypanosoma cruzi |
| H102A | kinetics severeyl impaired with substrate ribose 5-phosphate, but not affected with substrate ribulose 5-phosphate | Trypanosoma cruzi |
| H11A | 6fold increase in Km value, 8fold increase in kcat value, decrease in stability to freezing and thawing | Trypanosoma cruzi |
| H138A | little variations in kinetics compared to wild-type | Trypanosoma cruzi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-phospho-D-erythronate | - |
Trypanosoma cruzi |  |
| 4-phospho-D-erythronhydrazide | - |
Trypanosoma cruzi | |
| 4-phospho-D-erythronohydroxamic acid | competitive | Trypanosoma cruzi | |
| 5-phospho-D-ribonohydroxamic acid | - |
Trypanosoma cruzi | |
| iodoacetamide | 5 mM, complete inhibition | Trypanosoma cruzi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
D-ribulose 5-diphosphate | wild-type, pH 8.4, 25°C | Trypanosoma cruzi | |
| 2 | - |
D-Ribose 5-diphosphate | mutant H102A, pH 8.4, 25°C | Trypanosoma cruzi | |
| 2 | 3 | D-Ribose 5-diphosphate | muant H11A, pH 8.4, 25°C | Trypanosoma cruzi | |
| 2.5 | - |
D-ribulose 5-diphosphate | mutant H102A, pH 8.4, 25°C | Trypanosoma cruzi | |
| 4 | - |
D-Ribose 5-diphosphate | wild-type, pH 8.4, 25°C | Trypanosoma cruzi | |
| 10 | - |
D-Ribose 5-diphosphate | mutant H138A, pH 8.4, 25°C | Trypanosoma cruzi | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme is not metal-dependent | Trypanosoma cruzi |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17400 | - |
2 * 17400, calculated, 2 * 18000, SDS-PAGE | Trypanosoma cruzi |
| 18000 | - |
2 * 17400, calculated, 2 * 18000, SDS-PAGE | Trypanosoma cruzi |
| 39600 | - |
gel filtration | Trypanosoma cruzi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma cruzi | Q4CQE2 | type B enzyme | - |
| Trypanosoma cruzi CL Brener | Q4CQE2 | type B enzyme | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| amastigote | - |
Trypanosoma cruzi | - |
| epimastigote | highest expression | Trypanosoma cruzi | - |
| trypomastigote | metacyclic and in cell-culture | Trypanosoma cruzi | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribose 5-diphosphate | - |
Trypanosoma cruzi | D-ribulose 5-diphosphate | - |
r | |
| D-ribose 5-diphosphate | - |
Trypanosoma cruzi CL Brener | D-ribulose 5-diphosphate | - |
r | |
| D-ribulose 5-diphosphate | - |
Trypanosoma cruzi | D-ribose 5-diphosphate | - |
r | |
| D-ribulose 5-diphosphate | - |
Trypanosoma cruzi CL Brener | D-ribose 5-diphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 17400, calculated, 2 * 18000, SDS-PAGE | Trypanosoma cruzi |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
D-Ribose 5-diphosphate | mutant H102A, pH 8.4, 25°C | Trypanosoma cruzi | |
| 4.7 | - |
D-ribulose 5-diphosphate | wild-type, pH 8.4, 25°C | Trypanosoma cruzi | |
| 10 | - |
D-Ribose 5-diphosphate | mutant H138A, pH 8.4, 25°C | Trypanosoma cruzi | |
| 10.7 | - |
D-ribulose 5-diphosphate | mutant H102A, pH 8.4, 25°C | Trypanosoma cruzi | |
| 12 | - |
D-Ribose 5-diphosphate | wild-type, pH 8.4, 25°C | Trypanosoma cruzi | |
| 97 | - |
D-Ribose 5-diphosphate | muant H11A, pH 8.4, 25°C | Trypanosoma cruzi | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | broad | Trypanosoma cruzi |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
4-phospho-D-erythronohydroxamic acid | pH 8.4, 25°C | Trypanosoma cruzi | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| additional information | - |
IC50 values of 5-phospho-D-ribonohydroxamic acid and 4-phospho-D-erythronhydrazide are above 10 mM | Trypanosoma cruzi | additional information | |
| 0.7 | - |
pH 8.4, 25°C | Trypanosoma cruzi | 4-phospho-D-erythronohydroxamic acid | |
| 5 | - |
pH 8.4, 25°C | Trypanosoma cruzi | 4-phospho-D-erythronate | |
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Release 2023.1 (January 2023)
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