Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.1.6 extracted from

  • Jung, C.H.; Hartman, F.C.; Lu, T.Y.S.; Larimer, F.W.
    D-Ribose-5-phosphate isomerase from spinach: heterologous overexpression, purification, characterization, and site-directed mutagenesis of the recombinant enzyme (2000), Arch. Biochem. Biophys., 373, 409-417.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Spinacia oleracea

Protein Variants

Protein Variants Comment Organism
D87A turnover number with D-ribose-5-phosphate is 0.0012% of value for the the wild-type enzyme, moderate change in Km-value Spinacia oleracea
D90A turnover number with D-ribose-5-phosphate is 0.38% of value for the the wild-type enzyme, moderate change in Km-value Spinacia oleracea
E91A turnover number with D-ribose-5-phosphate is 27.5% of value for the the wild-type enzyme, moderate change in Km-value Spinacia oleracea
K100A turnover number with D-ribose-5-phosphate is 0.074% of value for the the wild-type enzyme, moderate change in Km-value Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
arabinose 5-phosphate
-
Spinacia oleracea
D-arabinose 5-phosphate effective Spinacia oleracea
phosphate competitive Spinacia oleracea
Xylulose 5-phosphate
-
Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.52
-
D-ribose-5-phosphate pH 8.0, 25°C, mutant enzyme D90A Spinacia oleracea
0.56
-
D-ribose-5-phosphate pH 8.0, 25°C, mutant enzyme D87A Spinacia oleracea
0.63
-
D-ribose-5-phosphate pH 8.0, 25°C, wild-type enzyme Spinacia oleracea
1.17
-
D-ribose-5-phosphate pH 8.0, 25°C, mutant enzyme E91A Spinacia oleracea
4.41
-
D-ribose-5-phosphate pH 8.0, 25°C, mutant enzyme K100A Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
2 * 25000, SDS-PAGE Spinacia oleracea
25066
-
2 * 25066, calculation from nucleotide sequence Spinacia oleracea
49000
-
gel filtration Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Spinacia oleracea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribose 5-phosphate
-
Spinacia oleracea D-ribulose 5-phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 25000, SDS-PAGE Spinacia oleracea
dimer 2 * 25066, calculation from nucleotide sequence Spinacia oleracea

Synonyms

Synonyms Comment Organism
RPI
-
Spinacia oleracea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0421
-
D-ribose-5-phosphate pH 8.0, 25°C, mutant enzyme D87A Spinacia oleracea
2.5 4 D-ribose-5-phosphate pH 8.0, 25°C, mutant enzyme K100A Spinacia oleracea
2.54
-
D-ribose-5-phosphate pH 8.0, 25°C, mutant enzyme K100A Spinacia oleracea
13.2
-
D-ribose-5-phosphate pH 8.0, 25°C, mutant enzyme D90A Spinacia oleracea
946
-
D-ribose-5-phosphate pH 8.0, 25°C, mutant enzyme E91A Spinacia oleracea
3440
-
D-ribose-5-phosphate pH 8.0, 25°C, wild-type enzyme Spinacia oleracea

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.7
-
arabinose 5-phosphate pH 8.0, 25°C Spinacia oleracea
4
-
Xylulose 5-phosphate pH 8.0, 25°C Spinacia oleracea
7.9
-
phosphate pH 8.0, 25°C Spinacia oleracea

pI Value

Organism Comment pI Value Maximum pI Value
Spinacia oleracea isoelectric focusing
-
5.1