Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Spinacia oleracea |
Protein Variants | Comment | Organism |
---|---|---|
D87A | turnover number with D-ribose-5-phosphate is 0.0012% of value for the the wild-type enzyme, moderate change in Km-value | Spinacia oleracea |
D90A | turnover number with D-ribose-5-phosphate is 0.38% of value for the the wild-type enzyme, moderate change in Km-value | Spinacia oleracea |
E91A | turnover number with D-ribose-5-phosphate is 27.5% of value for the the wild-type enzyme, moderate change in Km-value | Spinacia oleracea |
K100A | turnover number with D-ribose-5-phosphate is 0.074% of value for the the wild-type enzyme, moderate change in Km-value | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arabinose 5-phosphate | - |
Spinacia oleracea | |
D-arabinose 5-phosphate | effective | Spinacia oleracea | |
phosphate | competitive | Spinacia oleracea | |
Xylulose 5-phosphate | - |
Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
D-ribose-5-phosphate | pH 8.0, 25°C, mutant enzyme D90A | Spinacia oleracea | |
0.56 | - |
D-ribose-5-phosphate | pH 8.0, 25°C, mutant enzyme D87A | Spinacia oleracea | |
0.63 | - |
D-ribose-5-phosphate | pH 8.0, 25°C, wild-type enzyme | Spinacia oleracea | |
1.17 | - |
D-ribose-5-phosphate | pH 8.0, 25°C, mutant enzyme E91A | Spinacia oleracea | |
4.41 | - |
D-ribose-5-phosphate | pH 8.0, 25°C, mutant enzyme K100A | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
2 * 25000, SDS-PAGE | Spinacia oleracea |
25066 | - |
2 * 25066, calculation from nucleotide sequence | Spinacia oleracea |
49000 | - |
gel filtration | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribose 5-phosphate | - |
Spinacia oleracea | D-ribulose 5-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 25000, SDS-PAGE | Spinacia oleracea |
dimer | 2 * 25066, calculation from nucleotide sequence | Spinacia oleracea |
Synonyms | Comment | Organism |
---|---|---|
RPI | - |
Spinacia oleracea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0421 | - |
D-ribose-5-phosphate | pH 8.0, 25°C, mutant enzyme D87A | Spinacia oleracea | |
2.5 | 4 | D-ribose-5-phosphate | pH 8.0, 25°C, mutant enzyme K100A | Spinacia oleracea | |
2.54 | - |
D-ribose-5-phosphate | pH 8.0, 25°C, mutant enzyme K100A | Spinacia oleracea | |
13.2 | - |
D-ribose-5-phosphate | pH 8.0, 25°C, mutant enzyme D90A | Spinacia oleracea | |
946 | - |
D-ribose-5-phosphate | pH 8.0, 25°C, mutant enzyme E91A | Spinacia oleracea | |
3440 | - |
D-ribose-5-phosphate | pH 8.0, 25°C, wild-type enzyme | Spinacia oleracea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
arabinose 5-phosphate | pH 8.0, 25°C | Spinacia oleracea | |
4 | - |
Xylulose 5-phosphate | pH 8.0, 25°C | Spinacia oleracea | |
7.9 | - |
phosphate | pH 8.0, 25°C | Spinacia oleracea |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Spinacia oleracea | isoelectric focusing | - |
5.1 |