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Literature summary for 5.3.1.6 extracted from

  • Essenberg, M.K.; Cooper, R.A.
    Two ribose-5-phosphate isomerases from Escherichia coli K12: partial characterization of the enzymes and consideration of their possible physiological roles (1975), Eur. J. Biochem., 55, 323-332.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
iodoacetate 1.25 mM, 100% loss of ribosephosphate isomerase B, no effect on ribosephosphate isomerase A Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.83
-
 D-ribose 5-phosphate ribosephosphate isomerase B Escherichia coli
4.4
-
 D-ribose 5-phosphate ribosephosphate isomerase A Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000 34000 ribosephosphate isomerase B, gel filtration Escherichia coli
45000
-
 ribosephosphate isomerase A, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli the constitutive ribosephosphate isomerase A catalyzes the formation of ribose 5-phosphate from ribulose 5-phosphate and also participates in the reverse reaction during ribose and adenosine catabolism. The normal physiological role of the inducible ribosephosphate isomerase B is still uncertain ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 two enzyme forms: constitutive isomerase A and inducible isomerase B
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribose 5-phosphate
-
 Escherichia coli D-ribulose 5-phosphate
-
 ?
additional information the constitutive ribosephosphate isomerase A catalyzes the formation of ribose 5-phosphate from ribulose 5-phosphate and also participates in the reverse reaction during ribose and adenosine catabolism. The normal physiological role of the inducible ribosephosphate isomerase B is still uncertain Escherichia coli ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 30 min, ribosephosphate isomerase A retains 90% of its activity, ribosephosphate isomerase B retains 60% of its activity Escherichia coli
60
-
 ribosephosphate isomerase B: half-life 2.2 min, independent of enzyme and protein concentration Escherichia coli
60
-
 ribosephosphate isomerase A: complex dependence on protein concentration, at 1.0 mg/ml protein and greater, all activity is lost. At 0.05-0.75 mg/ml protein, 20-30% of the original activity is left after 30 min Escherichia coli