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Literature summary for 5.3.1.24 extracted from

  • Sterner, R.; Merz, A.; Thoma, R.; Kirschner, K.
    Phosphoribosylanthranilate isomerase and indoleglycerol-phosphate synthase: tryptophan biosynthetic enzymes from Thermotoga maritima (2001), Methods Enzymol., 331, 270-280.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
trpF gene is cloned and expressed in Escherichia coli Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment) Organism
x-ray structure Thermotoga maritima

General Stability

General Stability Organism
extremely stable enzyme Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00028
-
N-(5-phospho-beta-D-ribosyl)anthranilate pH 7.5, 25°C, dimer Thermotoga maritima
0.00039
-
N-(5-phospho-beta-D-ribosyl)anthranilate pH 7.5, 45°C, dimer Thermotoga maritima
0.00073
-
N-(5-phospho-beta-D-ribosyl)anthranilate pH 7.5, 60°C, dimer Thermotoga maritima
0.00103
-
N-(5-phospho-beta-D-ribosyl)anthranilate pH 7.5, 80°C, dimer Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23040
-
2 * 23040, calculated from the amino acid sequence Thermotoga maritima
30200
-
dimer, gel filtration Thermotoga maritima
49600
-
dimer, sedimentation equilibrium analysis Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-(5-phospho-beta-D-ribosyl)anthranilate Thermotoga maritima fourth step in tryptophan biosynthesis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
monofunctional enzyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant PRAI, expressed in Escherichia coli Thermotoga maritima

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.3
-
37°C Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-(5-phospho-beta-D-ribosyl)anthranilate
-
Thermotoga maritima 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
ir
N-(5-phospho-beta-D-ribosyl)anthranilate fourth step in tryptophan biosynthesis Thermotoga maritima 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 23040, calculated from the amino acid sequence Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
structural features resonsible for the high thermostability Thermotoga maritima
85
-
dimer, half-life: 310 min Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.7
-
N-(5-phospho-beta-D-ribosyl)anthranilate pH 7.5, 25°C, dimer Thermotoga maritima
13.5
-
N-(5-phospho-beta-D-ribosyl)anthranilate pH 7.5, 45°C, dimer Thermotoga maritima
38.5
-
N-(5-phospho-beta-D-ribosyl)anthranilate pH 7.5, 60°C, dimer Thermotoga maritima
130
-
N-(5-phospho-beta-D-ribosyl)anthranilate pH 7.5, 80°C, dimer Thermotoga maritima