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Literature summary for 5.3.1.24 extracted from
- Phosphoribosyl anthranilate isomerase catalyzes a reversible amadori reaction (1995), Biochemistry, 34, 5429-5439.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | competitive inhibitor | Escherichia coli |  |
| reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | competitive inhibitor | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0032 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | pH 7.5, 25°C, recombinant xPRAI, expressed in Escherichia coli | Saccharomyces cerevisiae | |
| 0.0047 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | pH 7.5 | Escherichia coli | |
| 0.0047 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | 25°C, monomeric domain PRAI[ML256-452] | Escherichia coli | |
| 0.0049 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | pH 7.5 | Escherichia coli | |
| 0.0049 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | 25°C, bifunctional IGPS:PRAI | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-(5-phospho-beta-D-ribosyl)anthranilate | Escherichia coli | third step in tryptophan biosynthesis | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
| N-(5-phospho-beta-D-ribosyl)anthranilate | Saccharomyces cerevisiae | third step in tryptophan biosynthesis | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
| N-(5-phospho-beta-D-ribosyl)anthranilate | Escherichia coli | involved in tryptophan biosynthesis from chorismate | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
| N-(5-phospho-beta-D-ribosyl)anthranilate | Saccharomyces cerevisiae | involved in tryptophan biosynthesis from chorismate | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
| N-(5-phospho-beta-D-ribosyl)anthranilate | Saccharomyces cerevisiae xPRAI | third step in tryptophan biosynthesis | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
| N-(5-phospho-beta-D-ribosyl)anthranilate | Saccharomyces cerevisiae xPRAI | involved in tryptophan biosynthesis from chorismate | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
bifunctional indole-3-glycerol-phosphate synthetase/PRA isomerase complex | - |
| Saccharomyces cerevisiae | - |
naturally monofunctional enzyme | - |
| Saccharomyces cerevisiae | - |
xPRAI | - |
| Saccharomyces cerevisiae xPRAI | - |
xPRAI | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| monomeric domain PRAI[ML256-452] and bifunctional IGPS:PRAI | Escherichia coli |
| recombinant xPRAI, expressed in Escherichia coli | Saccharomyces cerevisiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | kinetic mechanism | Escherichia coli | |
| N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | kinetic mechanism | Saccharomyces cerevisiae | |
| N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | catalytic mechanism | Escherichia coli | |
| N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | catalytic mechanism | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-(5-phospho-beta-D-ribosyl)anthranilate | mechanism | Escherichia coli | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | enolamine is the first product, which then tautomerizes to the alpha-amino ketone | r | |
| N-(5-phospho-beta-D-ribosyl)anthranilate | mechanism | Saccharomyces cerevisiae | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | enolamine is the first product, which then tautomerizes to the alpha-amino ketone | r | |
| N-(5-phospho-beta-D-ribosyl)anthranilate | thermodynamic equilibrium strongly favors the product | Saccharomyces cerevisiae | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | enolamine is the first product, which then tautomerizes to the alpha-amino ketone | r | |
| N-(5-phospho-beta-D-ribosyl)anthranilate | third step in tryptophan biosynthesis | Escherichia coli | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
| N-(5-phospho-beta-D-ribosyl)anthranilate | third step in tryptophan biosynthesis | Saccharomyces cerevisiae | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
| N-(5-phospho-beta-D-ribosyl)anthranilate | involved in tryptophan biosynthesis from chorismate | Escherichia coli | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
| N-(5-phospho-beta-D-ribosyl)anthranilate | involved in tryptophan biosynthesis from chorismate | Saccharomyces cerevisiae | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
| N-(5-phospho-beta-D-ribosyl)anthranilate | mechanism | Saccharomyces cerevisiae xPRAI | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | enolamine is the first product, which then tautomerizes to the alpha-amino ketone | r | |
| N-(5-phospho-beta-D-ribosyl)anthranilate | thermodynamic equilibrium strongly favors the product | Saccharomyces cerevisiae xPRAI | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | enolamine is the first product, which then tautomerizes to the alpha-amino ketone | r | |
| N-(5-phospho-beta-D-ribosyl)anthranilate | third step in tryptophan biosynthesis | Saccharomyces cerevisiae xPRAI | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
| N-(5-phospho-beta-D-ribosyl)anthranilate | involved in tryptophan biosynthesis from chorismate | Saccharomyces cerevisiae xPRAI | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PRAI[ML256-452] | engineered monofunctional variant of IGPS:PRAI, excised monomeric domain | Escherichia coli |
| PRAI[ML256-452] | engineered monofunctional variant of IGPS:PRAI, excised monomeric domain | Saccharomyces cerevisiae |
| xPRAI | monomeric variant of PRAI from Saccharomyces cerevisiae expressed in Escherichia coli | Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Escherichia coli |
| 25 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 32 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | pH 7.5 | Escherichia coli | |
| 32 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | 25°C, monomeric domain PRAI[ML256-452] | Escherichia coli | |
| 40 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | pH 7.5, 25°C | Escherichia coli | |
| 40 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | bifunctional IGPS:PRAI | Escherichia coli | |
| 69 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | pH 7.5, 25°C, recombinant xPRAI, expressed in E. coli | Saccharomyces cerevisiae | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0017 | - |
reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 25°C, recombinant xPRAI, expressed in Escherichia coli | Saccharomyces cerevisiae | |
| 0.0065 | - |
reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5 | Escherichia coli | |
| 0.0065 | - |
reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | 25°C, bifunctional IGPS:PRAI | Escherichia coli | |
| 0.0068 | - |
reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5 | Escherichia coli | |
| 0.0068 | - |
reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | 25°C, monomeric domain PRAI[ML256-452] | Escherichia coli | |
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