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Literature summary for 5.3.1.24 extracted from

  • Kirschner, K.; Szadkowski, H.; Jardetzky, T.S.; Hager, V.
    Phosphoribosylanthranilate isomerase-indoleglycerol-phosphate synthase from Escherichia coli (1987), Methods Enzymol., 142, 386-397.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Escherichia coli

General Stability

General Stability Organism
enzyme is resistant to protease attack Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
Acinetobacter calcoaceticus
reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
Bacillus subtilis
reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
[Brevibacterium] flavum
reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
Escherichia coli
reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
Pseudomonas putida
reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium
reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
Serratia marcescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
N-(5-phospho-beta-D-ribosyl)anthranilate pH 7.5, 20°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49370
-
1 * 49370, calculated from the amino acid sequence Escherichia coli
49400
-
1 * 49400, indole-3-glycerol-phosphate synthetase/PRA isomerase complex Salmonella enterica subsp. enterica serovar Typhimurium
49400
-
1 * 49400, indole-3-glycerol-phosphate synthetase/PRA isomerase complex Escherichia coli
49400
-
1 * 49400, indole-3-glycerol-phosphate synthetase/PRA isomerase complex Serratia marcescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-(5-phospho-beta-D-ribosyl)anthranilate Salmonella enterica subsp. enterica serovar Typhimurium fourth step in tryptophan biosynthesis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate Bacillus subtilis fourth step in tryptophan biosynthesis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate Escherichia coli fourth step in tryptophan biosynthesis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate Aspergillus nidulans fourth step in tryptophan biosynthesis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate Neurospora crassa fourth step in tryptophan biosynthesis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate Pseudomonas putida fourth step in tryptophan biosynthesis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate Acinetobacter calcoaceticus fourth step in tryptophan biosynthesis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate Serratia marcescens fourth step in tryptophan biosynthesis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate [Brevibacterium] flavum fourth step in tryptophan biosynthesis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate Salmonella enterica subsp. enterica serovar Typhimurium involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate Bacillus subtilis involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate Escherichia coli involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate Aspergillus nidulans involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate Neurospora crassa involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate Pseudomonas putida involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate Acinetobacter calcoaceticus involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate Serratia marcescens involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate [Brevibacterium] flavum involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate Escherichia coli W3110 / ATCC 27325 fourth step in tryptophan biosynthesis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate Escherichia coli W3110 / ATCC 27325 involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
not associated with indole-3-glycerol-phosphate synthetase
-
Aspergillus nidulans
-
multienzyme complex, in which one of the two different subunits carries three catalytic functions, including PRA isomerase and indole-3-glycerol-phosphate synthetase
-
Bacillus subtilis
-
not associated with indole-3-glycerol-phosphate synthetase
-
Escherichia coli
-
-
-
Escherichia coli
-
bifunctional indole-3-glycerol-phosphate synthetase/PRA isomerase complex
-
Escherichia coli W3110 / ATCC 27325
-
-
-
Neurospora crassa
-
multienzyme complex, in which one of the two different subunits carries three catalytic functions, including PRA isomerase and indole-3-glycerol-phosphate synthetase
-
Pseudomonas putida
-
not associated with indole-3-glycerol-phosphate synthetase
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
multifunctional indole-3-glycerol-phosphate synthetase/PRA isomerase complex
-
Serratia marcescens
-
bifunctional indole-3-glycerol-phosphate synthetase/PRA isomerase complex
-
[Brevibacterium] flavum
-
indole-3-glycerol-phosphate synthetase and PRA isomerase form an easily dissociable multienzyme complex
-

Oxidation Stability

Oxidation Stability Organism
very susceptible to autooxidation catalyzed by Cu2+ and other heavy metal ions, oxidation is prevented by EDTA and dithioerythritol Escherichia coli

Purification (Commentary)

Purification (Comment) Organism
indole-3-glycerol-phosphate synthetase/PRA isomerase complex Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.78
-
37°C Escherichia coli

Storage Stability

Storage Stability Organism
4°C, under argon, months, stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-(5-phospho-beta-D-ribosyl)anthranilate
-
Salmonella enterica subsp. enterica serovar Typhimurium 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
ir
N-(5-phospho-beta-D-ribosyl)anthranilate
-
Bacillus subtilis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
ir
N-(5-phospho-beta-D-ribosyl)anthranilate
-
Aspergillus nidulans 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
ir
N-(5-phospho-beta-D-ribosyl)anthranilate
-
Neurospora crassa 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
ir
N-(5-phospho-beta-D-ribosyl)anthranilate
-
Pseudomonas putida 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
ir
N-(5-phospho-beta-D-ribosyl)anthranilate
-
Acinetobacter calcoaceticus 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
ir
N-(5-phospho-beta-D-ribosyl)anthranilate
-
Serratia marcescens 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
ir
N-(5-phospho-beta-D-ribosyl)anthranilate
-
[Brevibacterium] flavum 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
ir
N-(5-phospho-beta-D-ribosyl)anthranilate structure of the indole-3-glycerol-phosphate synthetase/PRA isomerase complex, 2 well-defined functional domains: N-terminal indole-3-glycerol-phosphate synthetase, residues 1-255, and C-terminal PRA isomerase, residues 256-452, with limited noncovalent contacts between the domains, the two reactions are catalyzed independently from each other Escherichia coli 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
ir
N-(5-phospho-beta-D-ribosyl)anthranilate fourth step in tryptophan biosynthesis Salmonella enterica subsp. enterica serovar Typhimurium 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate fourth step in tryptophan biosynthesis Bacillus subtilis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate fourth step in tryptophan biosynthesis Escherichia coli 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate fourth step in tryptophan biosynthesis Aspergillus nidulans 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate fourth step in tryptophan biosynthesis Neurospora crassa 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate fourth step in tryptophan biosynthesis Pseudomonas putida 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate fourth step in tryptophan biosynthesis Acinetobacter calcoaceticus 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate fourth step in tryptophan biosynthesis Serratia marcescens 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate fourth step in tryptophan biosynthesis [Brevibacterium] flavum 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Salmonella enterica subsp. enterica serovar Typhimurium 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Bacillus subtilis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Escherichia coli 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Aspergillus nidulans 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Neurospora crassa 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Pseudomonas putida 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Acinetobacter calcoaceticus 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Serratia marcescens 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate [Brevibacterium] flavum 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate structure of the indole-3-glycerol-phosphate synthetase/PRA isomerase complex, 2 well-defined functional domains: N-terminal indole-3-glycerol-phosphate synthetase, residues 1-255, and C-terminal PRA isomerase, residues 256-452, with limited noncovalent contacts between the domains, the two reactions are catalyzed independently from each other Escherichia coli W3110 / ATCC 27325 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
ir
N-(5-phospho-beta-D-ribosyl)anthranilate fourth step in tryptophan biosynthesis Escherichia coli W3110 / ATCC 27325 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?
N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Escherichia coli W3110 / ATCC 27325 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 49400, indole-3-glycerol-phosphate synthetase/PRA isomerase complex Salmonella enterica subsp. enterica serovar Typhimurium
monomer 1 * 49400, indole-3-glycerol-phosphate synthetase/PRA isomerase complex Escherichia coli
monomer 1 * 49400, indole-3-glycerol-phosphate synthetase/PRA isomerase complex Serratia marcescens
monomer 1 * 49370, calculated from the amino acid sequence Escherichia coli
monomer separate monomeric enzyme Bacillus subtilis
monomer separate monomeric enzyme Pseudomonas putida
monomer separate monomeric enzyme Acinetobacter calcoaceticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
39
-
N-(5-phospho-beta-D-ribosyl)anthranilate pH 7.5, 20°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
assay at Escherichia coli