Protein Variants | Comment | Organism |
---|---|---|
D127N | turnover number for N-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 2481fold higher than that of the wild-type enzyme. The Km-value for N-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 3.3fold higher than that of the wild-type enzyme | Thermotoga maritima |
D51N | turnover number for N-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 2.3fold higher than that of the wild-type enzyme. The Km-value for N-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 9.4fold higher than that of the wild-type enzyme | Thermotoga maritima |
D8N | activity with N-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is immeasurable low | Thermotoga maritima |
H48A | turnover number for N-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 1.8fold lower than that of the wild-type enzyme. The Km-value for N-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 4.8fold lower than that of the wild-type enzyme | Thermotoga maritima |
R83N | turnover number for N-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 2.9fold higher than that of the wild-type enzyme. The Km-value for N-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 63.2fold higher than that of the wild-type enzyme | Thermotoga maritima |
T164A | turnover number for N-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 37.2fold higher than that of the wild-type enzyme. The Km-value for N-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 9.3fold higher than that of the wild-type enzyme | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 25°C or 37°C, pH 7.5, wild-type enzyme | Thermotoga maritima | |
1.6 | - |
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 25°C, pH 7.5 | Escherichia coli | |
1.8 | - |
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 37°C, pH 7.5 | Escherichia coli | |
2 | - |
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 25°C, pH 7.5, mutant enzyme D127N | Thermotoga maritima | |
2.9 | - |
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 25°C, pH 7.5, mutant enzyme H48A | Thermotoga maritima | |
5.6 | - |
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 25°C, pH 7.5, mutant enzyme T164A | Thermotoga maritima | |
6.4 | - |
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 25°C, pH 7.5, mutant enzyme D51N | Thermotoga maritima | |
37.9 | - |
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 25°C, pH 7.5, mutant enzyme R83N | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25600 | - |
analytical ultracentrifugation | Thermotoga maritima |
27400 | - |
analytical ultracentrifugation | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | the enzyme is involved in histidine biosynthesis | ? | - |
? | |
additional information | Thermotoga maritima | the enzyme is involved in histidine biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Thermotoga maritima | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | enzyme catalyzes Amadori rearrangement of a thermolabile aminoaldolase into the corresponding aminoketose, reaction mechanism involving general acid-base catalysis and a Schiff base intermediate is proposed | Escherichia coli | |
1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | enzyme catalyzes Amadori rearrangement of a thermolabile aminoaldolase into the corresponding aminoketose, reaction mechanism involving general acid-base catalysis and a Schiff base intermediate is proposed | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme catalyzes Amadori rearrangements of a thermolabile aminoaldolase into the corresponding aminoketose, reaction mechanism involving general acid-base catalysis and a Schiff base intermediate is poposed | Escherichia coli | ? | - |
? | |
additional information | the enzyme is involved in histidine biosynthesis | Escherichia coli | ? | - |
? | |
additional information | the enzyme is involved in histidine biosynthesis | Thermotoga maritima | ? | - |
? | |
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | - |
Escherichia coli | ? | - |
? | |
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | - |
Thermotoga maritima | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Escherichia coli |
monomer | - |
Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
eHisA | - |
Escherichia coli |
tHisA | - |
Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
10 min, 50% loss of the initial CD signal | Escherichia coli |
95 | - |
10 min, 50% loss of the initial CD signal | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00027 | - |
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 25°C, pH 7.5, mutant enzyme D127N | Thermotoga maritima | |
0.018 | - |
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 25°C, pH 7.5, mutant enzyme T164A | Thermotoga maritima | |
0.23 | - |
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 25°C, pH 7.5, mutant enzyme R83N | Thermotoga maritima | |
0.29 | - |
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 25°C, pH 7.5, mutant enzyme D51N | Thermotoga maritima | |
0.38 | - |
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 25°C, pH 7.5, mutant enzyme H48A | Thermotoga maritima | |
0.67 | - |
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 25°C, pH 7.5, wild-type enzyme | Thermotoga maritima | |
1.54 | - |
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 37°C, pH 7.5, wild-type enzyme | Thermotoga maritima | |
2.94 | - |
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 37°C, pH 7.5, wild-type enzyme | Thermotoga maritima | |
4.9 | - |
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 25°C, pH 7.5 | Escherichia coli | |
6.08 | - |
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 25°C, pH 7.5, wild-type enzyme | Thermotoga maritima | |
14.3 | - |
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 37°C, pH 7.5 | Escherichia coli | |
32 | - |
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 80°C, pH 7.5, wild-type enzyme | Thermotoga maritima |