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Literature summary for 5.3.1.16 extracted from

  • Henn-Sax, M.; Thoma, R.; Schmidt, S.; Hennig, M.; Kirschner, K.; Sterner, R.
    Two (betaalpha)8-barrel enzymes of histidine and tryptophan biosynthesis have similar reaction mechanisms and common strategies for protecting their labile substrates (2002), Biochemistry, 41, 12032-12042.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D127N turnover number for N‘-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 2481fold higher than that of the wild-type enzyme. The Km-value for N‘-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 3.3fold higher than that of the wild-type enzyme Thermotoga maritima
D51N turnover number for N‘-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 2.3fold higher than that of the wild-type enzyme. The Km-value for N‘-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 9.4fold higher than that of the wild-type enzyme Thermotoga maritima
D8N activity with N‘-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is immeasurable low Thermotoga maritima
H48A turnover number for N‘-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 1.8fold lower than that of the wild-type enzyme. The Km-value for N‘-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 4.8fold lower than that of the wild-type enzyme Thermotoga maritima
R83N turnover number for N‘-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 2.9fold higher than that of the wild-type enzyme. The Km-value for N‘-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 63.2fold higher than that of the wild-type enzyme Thermotoga maritima
T164A turnover number for N‘-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 37.2fold higher than that of the wild-type enzyme. The Km-value for N‘-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 9.3fold higher than that of the wild-type enzyme Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C or 37°C, pH 7.5, wild-type enzyme Thermotoga maritima
1.6
-
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5 Escherichia coli
1.8
-
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 37°C, pH 7.5 Escherichia coli
2
-
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, mutant enzyme D127N Thermotoga maritima
2.9
-
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, mutant enzyme H48A Thermotoga maritima
5.6
-
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, mutant enzyme T164A Thermotoga maritima
6.4
-
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, mutant enzyme D51N Thermotoga maritima
37.9
-
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, mutant enzyme R83N Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25600
-
analytical ultracentrifugation Thermotoga maritima
27400
-
analytical ultracentrifugation Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli the enzyme is involved in histidine biosynthesis ?
-
?
additional information Thermotoga maritima the enzyme is involved in histidine biosynthesis ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Thermotoga maritima
-
-
-

Reaction

Reaction Comment Organism Reaction ID
1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide enzyme catalyzes Amadori rearrangement of a thermolabile aminoaldolase into the corresponding aminoketose, reaction mechanism involving general acid-base catalysis and a Schiff base intermediate is proposed Escherichia coli
1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide enzyme catalyzes Amadori rearrangement of a thermolabile aminoaldolase into the corresponding aminoketose, reaction mechanism involving general acid-base catalysis and a Schiff base intermediate is proposed Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme catalyzes Amadori rearrangements of a thermolabile aminoaldolase into the corresponding aminoketose, reaction mechanism involving general acid-base catalysis and a Schiff base intermediate is poposed Escherichia coli ?
-
?
additional information the enzyme is involved in histidine biosynthesis Escherichia coli ?
-
?
additional information the enzyme is involved in histidine biosynthesis Thermotoga maritima ?
-
?
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
Escherichia coli ?
-
?
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
Thermotoga maritima ?
-
?

Subunits

Subunits Comment Organism
monomer
-
Escherichia coli
monomer
-
Thermotoga maritima

Synonyms

Synonyms Comment Organism
eHisA
-
Escherichia coli
tHisA
-
Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
10 min, 50% loss of the initial CD signal Escherichia coli
95
-
10 min, 50% loss of the initial CD signal Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00027
-
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, mutant enzyme D127N Thermotoga maritima
0.018
-
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, mutant enzyme T164A Thermotoga maritima
0.23
-
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, mutant enzyme R83N Thermotoga maritima
0.29
-
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, mutant enzyme D51N Thermotoga maritima
0.38
-
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, mutant enzyme H48A Thermotoga maritima
0.67
-
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, wild-type enzyme Thermotoga maritima
1.54
-
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 37°C, pH 7.5, wild-type enzyme Thermotoga maritima
2.94
-
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 37°C, pH 7.5, wild-type enzyme Thermotoga maritima
4.9
-
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5 Escherichia coli
6.08
-
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 25°C, pH 7.5, wild-type enzyme Thermotoga maritima
14.3
-
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 37°C, pH 7.5 Escherichia coli
32
-
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide 80°C, pH 7.5, wild-type enzyme Thermotoga maritima