Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermococcus onnurineus |
expression in Escherichia coli | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 90°C | Thermococcus onnurineus | |
0.68 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 90°C | Pyrococcus furiosus | |
1.2 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 25°C | Pyrococcus furiosus | |
1.67 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 25°C | Thermococcus onnurineus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
4 * 25000, SDS-PAGE | Thermococcus onnurineus |
25000 | - |
4 * 25000, SDS-PAGE | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | P62002 | - |
- |
Thermococcus onnurineus | B6YUE5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus onnurineus |
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glyceraldehyde 3-phosphate | - |
Thermococcus onnurineus | Glycerone phosphate | - |
? | |
D-Glyceraldehyde 3-phosphate | - |
Pyrococcus furiosus | Glycerone phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 25000, SDS-PAGE | Thermococcus onnurineus |
tetramer | 4 * 25000, SDS-PAGE | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
PfuTIM | - |
Pyrococcus furiosus |
TonTIM | - |
Thermococcus onnurineus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
no structural unfolding at 90°C | Thermococcus onnurineus |
90 | - |
no structural unfolding at 90°C | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1430000 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 25°C | Thermococcus onnurineus | |
1548000 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 25°C | Pyrococcus furiosus | |
25000000 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 90°C | Thermococcus onnurineus | |
57500000 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 90°C | Pyrococcus furiosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
857000 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 25°C | Thermococcus onnurineus | |
1269000 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 25°C | Pyrococcus furiosus | |
84880000 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 90°C | Pyrococcus furiosus | |
120400000 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 90°C | Thermococcus onnurineus |