Literature summary for 5.3.1.1 extracted from

Malabanan, M.M.; Amyes, T.L.; Richard, J.P.
Mechanism for activation of triosephosphate isomerase by phosphite dianion: the role of a ligand-driven conformational change (2011), J. Am. Chem. Soc., 133, 16428-16431.

Search for more literature for 5.3.1.1

Activating Compound

Activating Compound	Comment	Organism	Structure
phosphite	-	Trypanosoma brucei brucei

Protein Variants

Protein Variants	Comment	Organism
L232A	the mutation in a 6fold decrease in kcat/Km for the reversible isomerization of D-glyceraldehyde 3-phosphate to give dihydroxyacetone phosphate	Trypanosoma brucei brucei

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.077	-	dihydroxyacetone phosphate	mutant enzyme L232A, at pH 7.5 (30 mM triethanolamine), 25°C	Trypanosoma brucei brucei
0.14	-	D-glyceraldehyde 3-phosphate	mutant enzyme L232A, at pH 7.5 (30 mM triethanolamine), 25°C	Trypanosoma brucei brucei
0.25	-	D-glyceraldehyde 3-phosphate	wild type enzyme, at pH 7.5 (30 mM triethanolamine), 25°C	Trypanosoma brucei brucei
0.7	-	dihydroxyacetone phosphate	wild type enzyme, at pH 7.5 (30 mM triethanolamine), 25°C	Trypanosoma brucei brucei

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glyceraldehyde 3-phosphate	Trypanosoma brucei brucei	-	dihydroxyacetone phosphate	-	r
dihydroxyacetone phosphate	Trypanosoma brucei brucei	-	D-glyceraldehyde 3-phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei brucei	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate	-	Trypanosoma brucei brucei	dihydroxyacetone phosphate	-	r
dihydroxyacetone phosphate	-	Trypanosoma brucei brucei	D-glyceraldehyde 3-phosphate	-	r

Synonyms

Synonyms	Comment	Organism
TIM	-	Trypanosoma brucei brucei
Triosephosphate isomerase	-	Trypanosoma brucei brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.7	-	dihydroxyacetone phosphate	mutant enzyme L232A, at pH 7.5 (30 mM triethanolamine), 25°C	Trypanosoma brucei brucei
220	-	D-glyceraldehyde 3-phosphate	mutant enzyme L232A, at pH 7.5 (30 mM triethanolamine), 25°C	Trypanosoma brucei brucei
300	-	dihydroxyacetone phosphate	wild type enzyme, at pH 7.5 (30 mM triethanolamine), 25°C	Trypanosoma brucei brucei
2100	-	D-glyceraldehyde 3-phosphate	wild type enzyme, at pH 7.5 (30 mM triethanolamine), 25°C	Trypanosoma brucei brucei

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
61	-	dihydroxyacetone phosphate	mutant enzyme L232A, at pH 7.5 (30 mM triethanolamine), 25°C	Trypanosoma brucei brucei
430	-	dihydroxyacetone phosphate	wild type enzyme, at pH 7.5 (30 mM triethanolamine), 25°C	Trypanosoma brucei brucei
1500	-	D-glyceraldehyde 3-phosphate	mutant enzyme L232A, at pH 7.5 (30 mM triethanolamine), 25°C	Trypanosoma brucei brucei
8400	-	D-glyceraldehyde 3-phosphate	wild type enzyme, at pH 7.5 (30 mM triethanolamine), 25°C	Trypanosoma brucei brucei

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Release 2023.1 (January 2023)