Literature summary for 5.3.1.1 extracted from

Banerjee, M.; Balaram, H.; Joshi, N.V.; Balaram, P.
Engineered dimer interface mutants of triosephosphate isomerase: the role of inter-subunit interactions in enzyme function and stability (2011), Protein Eng. Des. Sel., 24, 463-472.

Search for more literature for 5.3.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli AA200 cells (a null mutant of inherent TIM gene)	Plasmodium falciparum

Protein Variants

Protein Variants	Comment	Organism
C13D	the mutant displays significant reduction in catalytic activity when compared with wild type enzyme (about 7.4fold decrease in kcat). The C13D mutant dissociates at concentrations above 1.25 mM	Plasmodium falciparum
C13E	the mutant displays significant reduction in catalytic activity when compared with wild type enzyme (about 3.3fold decrease in kcat). The C13E mutant retains dimeric at concentrations above 1.25 mM	Plasmodium falciparum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.31	-	D-glyceraldehyde 3-phosphate	mutant enzyme C13D, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication	Plasmodium falciparum
0.35	-	D-glyceraldehyde 3-phosphate	wild type enzyme, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication	Plasmodium falciparum
0.42	-	D-glyceraldehyde 3-phosphate	mutant enzyme C13E, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication	Plasmodium falciparum

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	Q07412	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Sephacryl-200 gel filtration	Plasmodium falciparum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate	-	Plasmodium falciparum	dihydroxyacetone phosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Plasmodium falciparum

Synonyms

Synonyms	Comment	Organism
TIM	-	Plasmodium falciparum
Triosephosphate isomerase	-	Plasmodium falciparum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	the melting temperature of the wild type enzyme is at 60°C	Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
600	-	D-glyceraldehyde 3-phosphate	mutant enzyme C13D, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication	Plasmodium falciparum
1367	-	D-glyceraldehyde 3-phosphate	mutant enzyme C13E, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication	Plasmodium falciparum
4467	-	D-glyceraldehyde 3-phosphate	wild type enzyme, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication	Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1933	-	D-glyceraldehyde 3-phosphate	mutant enzyme C13D, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication	Plasmodium falciparum
3250	-	D-glyceraldehyde 3-phosphate	mutant enzyme C13E, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication	Plasmodium falciparum
12750	-	D-glyceraldehyde 3-phosphate	wild type enzyme, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication	Plasmodium falciparum

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Release 2023.1 (January 2023)