Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of C-terminally His6-tagged TIM in Escherichia coli cytoplasm in a natively folded, enzymatically active form | Tenebrio molitor |
Crystallization (Comment) | Organism |
---|---|
purified recombinant C-terminally His6-tagged enzyme, sitting drop vapour diffusion method, room temperature, 0.003 ml of 18 mg/ml protein in 10 mM MES buffer, pH 6.5, and 15 mM NaCl, is mixed with 0.003 ml of reservoir solution containing 18% PEG MME 2000 as precipitant, X-ray diffraction structure determination and analysis at 2.0 A resolution | Tenebrio molitor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glycerol 1-phosphate | - |
Tenebrio molitor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.84 | - |
D-glyceraldehyde 3-phosphate | pH 7.0, 25°C | Tenebrio molitor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glyceraldehyde 3-phosphate | Tenebrio molitor | - |
Glycerone phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tenebrio molitor | Q8MPF2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged TIM from Escherichia coli by metal affinity chromatography | Tenebrio molitor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glyceraldehyde 3-phosphate | - |
Tenebrio molitor | Glycerone phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | gel filtration and SDS-PAGE, crystal structure, overview | Tenebrio molitor |
More | structure analysis and loop interactions of TIM, overview | Tenebrio molitor |
Synonyms | Comment | Organism |
---|---|---|
TIM | - |
Tenebrio molitor |
Triosephosphate isomerase | - |
Tenebrio molitor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Tenebrio molitor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4300 | - |
D-glyceraldehyde 3-phosphate | pH 7.0, 25°C | Tenebrio molitor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8.7 | - |
Tenebrio molitor |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
glycerol 1-phosphate | - |
Tenebrio molitor |
General Information | Comment | Organism |
---|---|---|
metabolism | triosephosphate isomerase is a ubiquitously distributed enzyme of the glycolysis pathway | Tenebrio molitor |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5119 | - |
D-glyceraldehyde 3-phosphate | pH 7.0, 25°C | Tenebrio molitor |