Crystallization (Comment) | Organism |
---|---|
determination of intrinsic fluorescence of wild-type and mutants lacking Trp residues, interpretation based on crystal structure. Fluorescence of all Trp residues is quenched by aromatic-aromatic interactions due to the proximity and orientation of the indole groups of Trp196 and Tro162. Quenching is also due to energy transfer to the charged resiudes that surround Trp75, Trp173, and Trp196 | Giardia intestinalis |
Protein Variants | Comment | Organism |
---|---|---|
F12W | mutant constructed for fluorescence studies, catalytic properties similar to wild-type | Giardia intestinalis |
W162F | mutant constructed for fluorescence studies, catalytic properties similar to wild-type | Giardia intestinalis |
W162F/W173F/W196F | mutant constructed for fluorescence studies, catalytic properties similar to wild-type | Giardia intestinalis |
W173F | mutant constructed for fluorescence studies, catalytic properties similar to wild-type | Giardia intestinalis |
W196F | mutant constructed for fluorescence studies, catalytic properties similar to wild-type | Giardia intestinalis |
W75F | mutant constructed for fluorescence quenching studies, catalytic properties similar to wild-type | Giardia intestinalis |
W75F/W162F/W173F | mutant constructed for fluorescence studies, catalytic properties similar to wild-type | Giardia intestinalis |
W75F/W162F/W196F | mutant constructed for fluorescence studies, catalytic properties similar to wild-type | Giardia intestinalis |
W75F/W173F/W196F | mutant constructed for fluorescence studies, catalytic properties similar to wild-type | Giardia intestinalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
D-glyceraldehyde 3-phosphate | mutant W162F | Giardia intestinalis | |
0.43 | - |
D-glyceraldehyde 3-phosphate | mutant W196F | Giardia intestinalis | |
0.6 | - |
D-glyceraldehyde 3-phosphate | mutant F12W | Giardia intestinalis | |
0.73 | - |
D-glyceraldehyde 3-phosphate | mutant W75F/W162F/W196F | Giardia intestinalis | |
0.74 | - |
D-glyceraldehyde 3-phosphate | mutant W75F | Giardia intestinalis | |
0.78 | - |
D-glyceraldehyde 3-phosphate | wildtype | Giardia intestinalis | |
0.94 | - |
D-glyceraldehyde 3-phosphate | mutant W173F | Giardia intestinalis | |
1 | - |
D-glyceraldehyde 3-phosphate | mutant W162F/W173F/W196F | Giardia intestinalis | |
1.12 | - |
D-glyceraldehyde 3-phosphate | mutant W75F/W162F/W173F | Giardia intestinalis | |
1.9 | - |
D-glyceraldehyde 3-phosphate | mutant W75F/W173F/W196F | Giardia intestinalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Giardia intestinalis | P36186 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate | - |
Giardia intestinalis | dihydroxyacetone phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3166 | - |
D-glyceraldehyde 3-phosphate | mutant W162F/W173F/W196F | Giardia intestinalis | |
3333 | - |
D-glyceraldehyde 3-phosphate | mutant W162F | Giardia intestinalis | |
3666 | - |
D-glyceraldehyde 3-phosphate | mutant W75F/W162F/W173F | Giardia intestinalis | |
4166 | - |
D-glyceraldehyde 3-phosphate | mutant W196F | Giardia intestinalis | |
4333 | - |
D-glyceraldehyde 3-phosphate | mutant W75F/W173F/W196F | Giardia intestinalis | |
5500 | - |
D-glyceraldehyde 3-phosphate | mutant F12W | Giardia intestinalis | |
6166 | - |
D-glyceraldehyde 3-phosphate | mutant W173F | Giardia intestinalis | |
7166 | - |
D-glyceraldehyde 3-phosphate | mutant W75F/W162F/W196F | Giardia intestinalis | |
7666 | - |
D-glyceraldehyde 3-phosphate | wildtype | Giardia intestinalis | |
8000 | - |
D-glyceraldehyde 3-phosphate | mutant W75F | Giardia intestinalis |