Crystallization (Comment) | Organism |
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structure of both a hexagonal and an orthorhombic crystal form to resolutions of 2.5 A and 2.3 A. In both crystal forms the enzyme exists as a tetramer of the (betaalpha)8-barrel. Hexagonal crystal form (crystallisation conditions 0.1 M HepesKOH (pH 7.5), 0.8 M sodium potassium phosphate) diffracts to 2.5 A and belongs to spacegroup P6(5)22/P6(1)22 with cell dimensions a = b = 186.9 A, c = 287.8 A, alpha = beta = 90°, gamma = 120°, suggesting two tetramers in the asymmetric unit. Orthorhombic crystals with cell dimensions a = 154.1 A, b = 91.0 A, c = 141.2 A, alpha = beta = gamma = 90° are obtained in 0.1 M sodium acetate (pH 4.6), 0.2 M ammonium sulfate, with PEG4000 at any concentration between 20% and 30% (w/v). Crystals grew within 48 hours at 20°C | Thermoproteus tenax |
Organism | UniProt | Comment | Textmining |
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Thermoproteus tenax | Q8NKN9 | - |
- |
Purification (Comment) | Organism |
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- |
Thermoproteus tenax |
Subunits | Comment | Organism |
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More | in solution the enzyme exhibits an equilibrium between inactive dimers and active tetramers | Thermoproteus tenax |
Synonyms | Comment | Organism |
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Triosephosphate isomerase | - |
Thermoproteus tenax |