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Literature summary for 5.2.1.8 extracted from

  • Boudko, S.P.; Ishikawa, Y.; Nix, J.; Chapman, M.S.; Baechinger, H.P.
    Structure of human peptidyl-prolyl cis-trans isomerase FKBP22 containing two EF-hand motifs (2014), Protein Sci., 23, 67-75.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene FKBP14, expression of His6-tagged wild-type and selenomethionine-labeled FKBP22 in Escherichia coli strains BL21(DE3) and B834(DE3), respectively Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant detagged wild-type and selenomethionine-labeled FKBP22, hanging drop vapor diffusion method, mixing of 0.002 ml of 3 mg/ml protein in 1 mM Tris/HCl, pH 7.2, and 0.05 mM CaCl2, with 0.001 ml of reservoir solution containing 0.1 M MES, 25% 1,2-propanediol, and 11-12% PEG 20000, pH 7.0-7.2, 1-2 weeks, X-ray diffraction structure determination and analysis at 1.9 A and 2.46 A resolution, respectively Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Homo sapiens 5783
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NWM8 gene FKBP14 encodes enzyme FKBP22
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged wild-type and selenomethionine-labeled FKBP22 from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography, tag cleavage by enterokinase, anion exchanchromatography, and dialysis Homo sapiens

Subunits

Subunits Comment Organism
dimer
-
Homo sapiens

Synonyms

Synonyms Comment Organism
FKBP22
-
Homo sapiens
Peptidyl-prolyl cis-trans isomerase
-
Homo sapiens

General Information

General Information Comment Organism
evolution the enzyme belongs to the FK506-binding protein (FKBP) family whose members are peptidyl-prolyl cis-trans isomerases with the enzymatic function attributed to the FKBP domain. Six members of this family localize to the mammalian endoplasmic reticulum. Four of them, FKBP22 (encoded by the FKBP14 gene), FKBP23 (FKBP7), FKBP60 (FKBP9), and FKBP65 (FKBP10), are unique among all FKBPs as they contain the EF-hand motifs. All FKBP-EFs contain an endoplasmic reticulum retention signal at the C-terminus Homo sapiens