Cloned (Comment) | Organism |
---|---|
gene FKBP14, expression of His6-tagged wild-type and selenomethionine-labeled FKBP22 in Escherichia coli strains BL21(DE3) and B834(DE3), respectively | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant detagged wild-type and selenomethionine-labeled FKBP22, hanging drop vapor diffusion method, mixing of 0.002 ml of 3 mg/ml protein in 1 mM Tris/HCl, pH 7.2, and 0.05 mM CaCl2, with 0.001 ml of reservoir solution containing 0.1 M MES, 25% 1,2-propanediol, and 11-12% PEG 20000, pH 7.0-7.2, 1-2 weeks, X-ray diffraction structure determination and analysis at 1.9 A and 2.46 A resolution, respectively | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NWM8 | gene FKBP14 encodes enzyme FKBP22 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type and selenomethionine-labeled FKBP22 from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography, tag cleavage by enterokinase, anion exchanchromatography, and dialysis | Homo sapiens |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
FKBP22 | - |
Homo sapiens |
Peptidyl-prolyl cis-trans isomerase | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the FK506-binding protein (FKBP) family whose members are peptidyl-prolyl cis-trans isomerases with the enzymatic function attributed to the FKBP domain. Six members of this family localize to the mammalian endoplasmic reticulum. Four of them, FKBP22 (encoded by the FKBP14 gene), FKBP23 (FKBP7), FKBP60 (FKBP9), and FKBP65 (FKBP10), are unique among all FKBPs as they contain the EF-hand motifs. All FKBP-EFs contain an endoplasmic reticulum retention signal at the C-terminus | Homo sapiens |