Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyclic CRYPEVEIC | the cyclic peptide is specific for the active site of the PPIase domain | Homo sapiens | |
linear CRYPEVEIC | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose column chromatography and S-Sepharose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-Ala-Glu-(cis)-Pro-Phe-4-nitroanilide | - |
Homo sapiens | succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide | - |
? | |
Trp-Phe-Tyr-pSer-Pro-Arg-4-nitroanilide | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
peptidylprolyl isomerase | - |
Homo sapiens |
Pin1 | - |
Homo sapiens |
PPIase | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
cyclic CRYPEVEIC | using Trp-Phe-Tyr-pSer-Pro-Arg-4-nitroanilide as substrate, in 50 mM HEPES, 0.1 M NaCl, 5 mM NaN3, pH 7.4, at 22°C | Homo sapiens | |
0.00052 | - |
cyclic CRYPEVEIC | using succinyl-Ala-Glu-(cis)-Pro-Phe-4-nitroanilide as substrate, in 50 mM HEPES, 0.1 M NaCl, 5 mM NaN3, pH 7.4, at 22°C | Homo sapiens | |
0.044 | - |
linear CRYPEVEIC | using succinyl-Ala-Glu-(cis)-Pro-Phe-4-nitroanilide as substrate, in 50 mM HEPES, 0.1 M NaCl, 5 mM NaN3, pH 7.4, at 22°C | Homo sapiens |