Literature summary for 5.2.1.8 extracted from

Xu, L.R.; Yan, X.; Luo, M.; Guan, Y.X.; Yao, S.J.
Preparation, characterization and refolding in vitro of a recombinant human cyclophilin A mutant: effect of a single Pro/Ser substitution on cyclophilin A structure and properties (2008), Biotechnol. Prog., 24, 302-310.

Search for more literature for 5.2.1.8

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
P16S	10fold decrease in ratio kcat/Km at 10°C. Mutant is extremely sensitive to guanidinium-HCl and shows increased susceptibility to urea. Folding time of the mutant is extended	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
cyclosporin A	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	isoform cyclophilin A	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme and mutant P16S	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-succinyl-Ala-Ala-(trans)-Pro-Phe-4-nitroanilide	-	Homo sapiens	N-succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide	-	?

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00000265	-	-	Homo sapiens	cyclosporin A

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Release 2023.1 (January 2023)