Protein Variants | Comment | Organism |
---|---|---|
E178V | slightly more active than wild-type enzyme with succinyl-Ala-Phe-Pro-Phe-4-nitroanilide, as active as wild-type enzyme in refolding of reduced and carboxymethylated RNAse T1, chaperone activity comparable with wild-type, not impaired in association with nascent proteins | Escherichia coli |
F198A | inactive with succinyl-Ala-Phe-Pro-Phe-4-nitroanilide, inactive in refolding of reduced and carboxymethylated RNAse T1, chaperone activity comparable with wild-type, not impaired in association with nascent proteins | Escherichia coli |
F233L | inactive with succinyl-Ala-Phe-Pro-Phe-4-nitroanilide, less active than wild-type enzyme in refolding of reduced and carboxymethylated RNAse T1, chaperone activity comparable with wild-type, not impaired in association with nascent proteins | Escherichia coli |
Y221F | mutant enzyme shows 15% of the activity of the wild-type enzyme with succinyl-Ala-Phe-Pro-Phe-4-nitroanilide as substrate, less active than wild-type enzyme in refolding of reduced and carboxymethylated RNAse T1, chaperone activity comparable with wild-type, not impaired in association with nascent proteins | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
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Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | trigger factor's peptidyl-prolyl cis/trans isomerase activity is not essential for the folding of cytosolic proteins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | trigger factor's peptidyl-prolyl cis/trans isomerase activity is not essential for the folding of cytosolic proteins | Escherichia coli | ? | - |
? | |
RNAse T1 | reduced and carboxymethylated RNAse T1, refolding by trans to cis isomerization of peptidyl-prolyl bonds at Pro39 and Pro55 | Escherichia coli | ? | - |
? | |
succinyl-Ala-Phe-(trans)-Pro-Phe-4-nitroanilide | - |
Escherichia coli | succinyl-Ala-Phe-(cis)-Pro-Phe-4-nitroanilide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TF | - |
Escherichia coli |
trigger factor | - |
Escherichia coli |