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Literature summary for 5.2.1.8 extracted from

  • Bang, H.; Pecht, A.; Raddatz, G.; Scior, T.; Solbach, W.; Brune, K.; Pahl, A.
    Prolyl isomerases in a minimal cell. Catalysis of protein folding by trigger factor from Mycoplasma genitalium (2000), Eur. J. Biochem., 267, 3270-3280.
    View publication on PubMed
Search for more literature for 5.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycoplasma genitalium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12440
-
 peptidylprolyl isomerase domain, N-terminal sequence analysis Mycoplasma genitalium
52330
-
 trigger factor, N-terminal sequence analysis Mycoplasma genitalium

Organism

Organism UniProt Comment Textmining
Mycoplasma genitalium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycoplasma genitalium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-(trans)-Pro-Phe
-
 Mycoplasma genitalium Ala-Ala-(cis)-Pro-Phe
-
 r
Ala-Ala-Ala-(trans)-Pro-Phe
-
 Mycoplasma genitalium Ala-Ala-Ala-(cis)-Pro-Phe
-
 r
additional information neither the active domain nor the intact protein can catalyze the cis/trans isomerization of the tripeptide Ala-Pro-Phe, less than 5% of the activity with succinyl-Ala-Ala-Pro-Phe-p-nitroanilide is observed with: succinyl-Ala-Gly-Pro-Phe-p-nitroanilide, succinyl-Ala-Lys-Pro-Phe-p-nitroanilide, succinyl-Ala-Glu-Pro-Phe-p-nitroanilide. The organism is devoid of all known peptidyl-prolyl cis/trans isomerases except the trigger factor. The trigger factor shows dual function as chaperone and prolyl isomerase Mycoplasma genitalium ?
-
 ?
succinyl-Ala-Ala-(trans)-Pro-Phe-4-nitroanilide
-
 Mycoplasma genitalium succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide
-
 r
succinyl-Ala-His-(trans)-Pro-Phe-4-nitroanilide
-
 Mycoplasma genitalium succinyl-Ala-His-(cis)-Pro-Phe-4-nitroanilide
-
 r
succinyl-Ala-Leu-(trans)-Pro-Phe-4-nitroanilide
-
 Mycoplasma genitalium succinyl-Ala-Leu-(cis)-Pro-Phe-4-nitroanilide
-
 r
succinyl-Ala-Phe-(trans)-Pro-Phe-4-nitroanilide
-
 Mycoplasma genitalium succinyl-Ala-Phe-(cis)-Pro-Phe-4-nitroanilide
-
 r

Synonyms

Synonyms Comment Organism
trigger factor
-
 Mycoplasma genitalium