Literature summary for 5.2.1.8 extracted from

Liu, J.; Albers, M.W.; Chen, C.M.; Schreiber, S.L.; Walsh, C.T.
Cloning, expression, and purification of human cyclophilin in Escherichia coli and assessment of the catalytic role of cysteines by site-directed mutagenesis (1990), Proc. Natl. Acad. Sci. USA, 87, 2304-2308.

Search for more literature for 5.2.1.8

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
C115A	Cys at position 52, 62, 115, and 161 are mutated individually to Ala and the purified mutant proteins to retain full affinity for cyclosporin A and equivalent catalytic efficiency as a rotamase	Homo sapiens
C161A	Cys at position 52, 62, 115, and 161 are mutated individually to Ala and the purified mutant proteins to retain full affinity for cyclosporin A and equivalent catalytic efficiency as a rotamase	Homo sapiens
C52A	Cys at position 52, 62, 115, and 161 are mutated individually to Ala and the purified mutant proteins to retain full affinity for cyclosporin A and equivalent catalytic efficiency as a rotamase	Homo sapiens
C62A	Cys at position 52, 62, 115, and 161 are mutated individually to Ala and the purified mutant proteins to retain full affinity for cyclosporin A and equivalent catalytic efficiency as a rotamase	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
peptidylproline (omega=180) = peptidylproline (omega=0)	mechanism that involves distortion of bound substrate with a twisted, 90° , peptidyl-propyl amide bond	Homo sapiens	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
JURKAT cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide	-	Homo sapiens	succinyl-Ala-Ala-(trans)-Pro-Phe 4-nitroanilide	-	?

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Release 2023.1 (January 2023)