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Literature summary for 5.1.3.8 extracted from

  • Takahashi, S.; Takahashi, K.; Kaneko, T.; Ogasawara, H.; Shindo, S.; Kobayashi, M.
    Human renin-binding protein is the enzyme N-acetyl-D-glucosamine 2-epimerase (1999), J. Biochem., 125, 348-353.
    View publication on PubMed
Search for more literature for 5.1.3.8

Activating Compound

Activating Compound Comment Organism Structure
ATP required, Km: 0.13 mM Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 under the transcriptional control of taq promoter Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid) 0.1 mM, more than 95% inhibition Homo sapiens
Monoiodoacetic acid 10 mM, more than 95% inhibition Homo sapiens
NEM 1 mM, more than 95% inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12.8
-
 N-acetyl-D-mannosamine 37°C, pH 7.5 Homo sapiens
21.3
-
 N-acetyl-D-glucosamine 37°C, pH 7.5 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 2 * 45000, SDS-PAGE Homo sapiens
85000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35.8
-
 37°C, pH 7.5 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with mannosamine, glucosamine, mannose, glucose and N-acetyl-galactosamine Homo sapiens ?
-
 ?
additional information the enzyme inhibits porcine renin activity through formation of a complex of porcine renin with the recombinant enzyme, the so-called high-molecular-weight renin Homo sapiens ?
-
 ?
N-acetyl-D-mannosamine
-
 Homo sapiens N-acetyl-D-glucosamine
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 45000, SDS-PAGE Homo sapiens