Cloned (Comment) | Organism |
---|---|
expression of the enzyme obtained from HB8 strain in Escherichia coli as His-tagged protein | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
determined by SDS-PAGE and MALDI-TOF mass spectrometry | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Thermus thermophilus | Thermus thermophilus HB8 show dual functions for catalyzing conversion of UDP-D-glucose to UDP-D-galactose and between their N-acetylated forms | ? | - |
? | |
UDP-N-acetylgalactosamine | Thermus thermophilus | GalE epimerizes UDP-N-acetylgalactosamine in a dose-dependent manner | UDP-N-acetylglucosamine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by using nickel-NTA affinity chromatography | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Thermus thermophilus HB8 show dual functions for catalyzing conversion of UDP-D-glucose to UDP-D-galactose and between their N-acetylated forms | Thermus thermophilus | ? | - |
? | |
UDP-N-acetylgalactosamine | GalE epimerizes UDP-N-acetylgalactosamine in a dose-dependent manner | Thermus thermophilus | UDP-N-acetylglucosamine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GalE | - |
Thermus thermophilus |
UDP-N-acetylglucosamine-4'-epimerase | - |
Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
treatment at 80 °C for 30 min reduces the enzyme activity by 78% | Thermus thermophilus |
90 | - |
enzyme is stable at 90°C; after incubating at this temperature for 10 min, 90% of the enzymatic activity remains | Thermus thermophilus |
Organism | Comment | Expression |
---|---|---|
Thermus thermophilus | overexpression in Thermus thermophilus HB27 leads to an increased capacity of biofilm production | up |
General Information | Comment | Organism |
---|---|---|
physiological function | gene is important to biofilm formation because of its involvement in epimerizing UDP-N-acetylgalactosamine for exopolysaccharide biosynthesis | Thermus thermophilus |