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Literature summary for 5.1.3.7 extracted from

  • Niou, Y.K.; Wu, W.L.; Lin, L.C.; Yu, M.S.; Shu, H.Y.; Yang, H.H.; Lin, G.H.
    Role of galE on biofilm formation by Thermus spp. (2009), Biochem. Biophys. Res. Commun., 390, 313-318.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of the enzyme obtained from HB8 strain in Escherichia coli as His-tagged protein Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
determined by SDS-PAGE and MALDI-TOF mass spectrometry Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Thermus thermophilus Thermus thermophilus HB8 show dual functions for catalyzing conversion of UDP-D-glucose to UDP-D-galactose and between their N-acetylated forms ?
-
?
UDP-N-acetylgalactosamine Thermus thermophilus GalE epimerizes UDP-N-acetylgalactosamine in a dose-dependent manner UDP-N-acetylglucosamine
-
r

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by using nickel-NTA affinity chromatography Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Thermus thermophilus HB8 show dual functions for catalyzing conversion of UDP-D-glucose to UDP-D-galactose and between their N-acetylated forms Thermus thermophilus ?
-
?
UDP-N-acetylgalactosamine GalE epimerizes UDP-N-acetylgalactosamine in a dose-dependent manner Thermus thermophilus UDP-N-acetylglucosamine
-
r

Synonyms

Synonyms Comment Organism
GalE
-
Thermus thermophilus
UDP-N-acetylglucosamine-4'-epimerase
-
Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
treatment at 80 °C for 30 min reduces the enzyme activity by 78% Thermus thermophilus
90
-
enzyme is stable at 90°C; after incubating at this temperature for 10 min, 90% of the enzymatic activity remains Thermus thermophilus

Expression

Organism Comment Expression
Thermus thermophilus overexpression in Thermus thermophilus HB27 leads to an increased capacity of biofilm production up

General Information

General Information Comment Organism
physiological function gene is important to biofilm formation because of its involvement in epimerizing UDP-N-acetylgalactosamine for exopolysaccharide biosynthesis Thermus thermophilus