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Literature summary for 5.1.3.7 extracted from

  • Valiente, E.; Jimenez, N.; Merino, S.; Tomas, J.M.; Amaro, C.
    Vibrio vulnificus biotype 2 serovar E gne but not galE is essential for lipopolysaccharide biosynthesis and virulence (2008), Infect. Immun., 76, 1628-1638.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine mutant deficient for gne shows increased surface hydrophobicity, produces deep alterations in the outer membrane architecture, and displays noticeable increases in the sensitivity to microcidal peptides, to eel and human sera, and to phagocytosis/opsonophagocytosis. Significant attenuation of virulence for eels and mice is observed with the mutant, which is correlated with the loss of the O-antigen lipopolysaccharide, while the capsule is maintained Vibrio vulnificus

Protein Variants

Protein Variants Comment Organism
additional information a gne-deficient mutant completely lacks UDPGalNAc 4-epimerase activity and has UDP-Gal 4-epimerase activity in after growth on D-glucose or D-galactose that is not significantly different from that of the wild-type strain. Mutation increases the surface hydrophobicity, produces deep alterations in the outer membrane architecture, and results in noticeable increases in the sensitivity to microcidal peptides, to eel and human sera, and to phagocytosis/opsonophagocytosis. Significant attenuation of virulence for eels and mice is observed with the mutant Vibrio vulnificus

Organism

Organism UniProt Comment Textmining
Vibrio vulnificus Q7MH46 biotype 2 serovar E, isoform Gne
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0119
-
growth on D-galactose as sole carbon source Vibrio vulnificus
0.0206
-
growth on D-glucose as sole carbon source Vibrio vulnificus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine
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Vibrio vulnificus UDP-N-acetyl-D-galactosamine
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r