Cloned (Comment) | Organism |
---|---|
recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21-Gold (DE3) | Aspergillus nidulans |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme in complex with UDP-galactose using the microbatch method, 15 mg/ml protein in 25 mM Tris, pH 8, is used with crystallization solution containing 20% w/v PEG 3350, 0.1 M Bis-Tris propane, pH 7.5, and 0.2 M sodium fluoride, 2 weeks, X-ray diffraction structure determination and analysis at 2.4 A resolution | Aspergillus nidulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose | Aspergillus nidulans | the NAD+-dependent enzyme is responsible for reversibly inverting the 4-hydroxyl configuration of UDP-alpha-D-galactose to form UDP-alpha-D-glucose | UDP-alpha-D-galactose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | C8VAU8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21-Gold (DE3) | Aspergillus nidulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose | - |
Aspergillus nidulans | UDP-alpha-D-galactose | - |
r | |
UDP-alpha-D-glucose | the NAD+-dependent enzyme is responsible for reversibly inverting the 4-hydroxyl configuration of UDP-alpha-D-galactose to form UDP-alpha-D-glucose | Aspergillus nidulans | UDP-alpha-D-galactose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GalE | - |
Aspergillus nidulans |
UDP-galactose-4-epimerase | - |
Aspergillus nidulans |
UDP-glucose-4-epimerase | - |
Aspergillus nidulans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on | Aspergillus nidulans |