Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus horikoshii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 5 mM, 95% loss of activity | Pyrococcus horikoshii | |
Dimethylsulfoxide | 10%, 26% inhibition | Pyrococcus horikoshii | |
methanol | 10%, 26% inhibition | Pyrococcus horikoshii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
UDP-alpha-D-galactose | pH 6.5, 60°C | Pyrococcus horikoshii | |
2.24 | - |
UDP-alpha-D-glucose | pH 6.5, 60°C | Pyrococcus horikoshii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 5 mM, activity is increased by 30% | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Pyrococcus horikoshii |
70000 | - |
gel filtration | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O73960 | - |
- |
Pyrococcus horikoshii OT-3 | O73960 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-galactose | - |
Pyrococcus horikoshii | UDP-alpha-D-glucose | - |
? | |
UDP-alpha-D-galactose | - |
Pyrococcus horikoshii OT-3 | UDP-alpha-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 35000, SDS-PAGE | Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
GalE | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Pyrococcus horikoshii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
1 h, stable up to | Pyrococcus horikoshii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
UDP-alpha-D-galactose | pH 6.5, 60°C | Pyrococcus horikoshii | |
1.1 | - |
UDP-alpha-D-glucose | pH 6.5, 60°C | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Pyrococcus horikoshii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 80% of maximal activit, pH 9.0: about 50% of maximal activity | Pyrococcus horikoshii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | highly stable | Pyrococcus horikoshii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.49 | - |
UDP-alpha-D-glucose | pH 6.5, 60°C | Pyrococcus horikoshii | |
4.34 | - |
UDP-alpha-D-galactose | pH 6.5, 60°C | Pyrococcus horikoshii |