Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus anthracis |
gene BAS5114, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) | Bacillus anthracis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Bacillus anthracis | |
Mg2+ | 4 mM are included in assay medium | Bacillus anthracis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36900 | - |
predicted by amino acid sequence | Bacillus anthracis |
37800 | - |
predicted by amino acid sequence | Bacillus anthracis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bacillus anthracis | the gene encodes a bifunctional enzyme with both UDP-GlcNAc 4-epimerase and UDP-Glc 4-epimerase activities and that no other annotated UDP-Glc 4-epimerase gene encodes a UDP-GlcNAc 4-epimerase | ? | - |
? | |
additional information | Bacillus anthracis | UDP-N-acetylgalactoseamine 4-epimerase encoded by gene BAS5304 also shows UDP-glucose 4-epimerase activity, overview | ? | - |
? | |
UDP-alpha-D-glucose | Bacillus anthracis | - |
UDP-alpha-D-galactose | - |
r | |
UDP-galactose | Bacillus anthracis | - |
UDP-glucose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | Q81JK4 | - |
- |
Bacillus anthracis | Q81K34 | - |
- |
Purification (Comment) | Organism |
---|---|
by using nickel-nitrilotriacetic acid affinity chromatography and gel filtration | Bacillus anthracis |
recombinant enzyme from Escherichia coli strain BL21(DE3) | Bacillus anthracis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the gene encodes a bifunctional enzyme with both UDP-GlcNAc 4-epimerase and UDP-Glc 4-epimerase activities and that no other annotated UDP-Glc 4-epimerase gene encodes a UDP-GlcNAc 4-epimerase | Bacillus anthracis | ? | - |
? | |
additional information | UDP-N-acetylgalactoseamine 4-epimerase encoded by gene BAS5304 also shows UDP-glucose 4-epimerase activity, overview | Bacillus anthracis | ? | - |
? | |
additional information | no activity with UDP-N-acetylgalactoseamine | Bacillus anthracis | ? | - |
? | |
UDP-alpha-D-glucose | - |
Bacillus anthracis | UDP-alpha-D-galactose | - |
r | |
UDP-galactose | - |
Bacillus anthracis | UDP-glucose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
UDP-Glc 4-epimerase | - |
Bacillus anthracis |
UDP-GlcNAc 4-epimerase | - |
Bacillus anthracis |
UDP-glucose 4-epimerase | - |
Bacillus anthracis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus anthracis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
5 min, purified recombinant enzyme, inactivation | Bacillus anthracis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Bacillus anthracis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | contains an NAD+ binding site | Bacillus anthracis | |
NAD+ | - |
Bacillus anthracis |