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Literature summary for 5.1.3.2 extracted from

  • Dong, S.; Chesnokova, O.N.; Turnbough, C.L.; Pritchard, D.G.
    Identification of the UDP-N-acetylglucosamine 4-epimerase involved in exosporium protein glycosylation in Bacillus anthracis (2009), J. Bacteriol., 191, 7094-7101.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus anthracis
gene BAS5114, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) Bacillus anthracis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Bacillus anthracis
Mg2+ 4 mM are included in assay medium Bacillus anthracis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36900
-
predicted by amino acid sequence Bacillus anthracis
37800
-
predicted by amino acid sequence Bacillus anthracis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus anthracis the gene encodes a bifunctional enzyme with both UDP-GlcNAc 4-epimerase and UDP-Glc 4-epimerase activities and that no other annotated UDP-Glc 4-epimerase gene encodes a UDP-GlcNAc 4-epimerase ?
-
?
additional information Bacillus anthracis UDP-N-acetylgalactoseamine 4-epimerase encoded by gene BAS5304 also shows UDP-glucose 4-epimerase activity, overview ?
-
?
UDP-alpha-D-glucose Bacillus anthracis
-
UDP-alpha-D-galactose
-
r
UDP-galactose Bacillus anthracis
-
UDP-glucose
-
r

Organism

Organism UniProt Comment Textmining
Bacillus anthracis Q81JK4
-
-
Bacillus anthracis Q81K34
-
-

Purification (Commentary)

Purification (Comment) Organism
by using nickel-nitrilotriacetic acid affinity chromatography and gel filtration Bacillus anthracis
recombinant enzyme from Escherichia coli strain BL21(DE3) Bacillus anthracis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the gene encodes a bifunctional enzyme with both UDP-GlcNAc 4-epimerase and UDP-Glc 4-epimerase activities and that no other annotated UDP-Glc 4-epimerase gene encodes a UDP-GlcNAc 4-epimerase Bacillus anthracis ?
-
?
additional information UDP-N-acetylgalactoseamine 4-epimerase encoded by gene BAS5304 also shows UDP-glucose 4-epimerase activity, overview Bacillus anthracis ?
-
?
additional information no activity with UDP-N-acetylgalactoseamine Bacillus anthracis ?
-
?
UDP-alpha-D-glucose
-
Bacillus anthracis UDP-alpha-D-galactose
-
r
UDP-galactose
-
Bacillus anthracis UDP-glucose
-
r

Synonyms

Synonyms Comment Organism
UDP-Glc 4-epimerase
-
Bacillus anthracis
UDP-GlcNAc 4-epimerase
-
Bacillus anthracis
UDP-glucose 4-epimerase
-
Bacillus anthracis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bacillus anthracis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
5 min, purified recombinant enzyme, inactivation Bacillus anthracis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Bacillus anthracis

Cofactor

Cofactor Comment Organism Structure
additional information contains an NAD+ binding site Bacillus anthracis
NAD+
-
Bacillus anthracis