Literature summary for 5.1.3.2 extracted from

Bernatchez, S.; Szymanski, C.M.; Ishiyama, N.; Li, J.; Jarrell, H.C.; Lau, P.C.; Berghuis, A.M.; Young, N.M.; Wakarchuk, W.W.
A single bifunctional UDP-GlcNAc/Glc 4-epimerase supports the synthesis of three cell surface glycoconjugates in Campylobacter jejuni (2005), J. Biol. Chem., 280, 4792-4802.

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Cloned(Commentary)

Cloned (Comment)	Organism
overproduction of MalE-Gne fusion protein in Escherichia coli	Campylobacter jejuni

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.78	-	UDP-glucose	37°C	Campylobacter jejuni
0.784	-	UDP-galactose	37°C	Campylobacter jejuni
1.07	-	UDP-GalNAc	37°C	Campylobacter jejuni
1.087	-	UDP-GlcNAc	37°C	Campylobacter jejuni

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-galactose	Campylobacter jejuni	the bifunctional enzyme UDP-GlcNAc/Glc 4-epimerase is the sole supplier of the UDP-galactose and UDP-GalNAc required for synthesis of lipooligosaccharide	UDP-glucose	-	r
UDP-GalNAc	Campylobacter jejuni	the bifunctional enzyme UDP-GlcNAc/Glc 4-epimerase is the sole supplier of the UDP-galactose and UDP-GalNAc required for synthesis of lipooligosaccharide. The enzyme supplies the UDP-GalNAc required for the synthesis of the capsule	UDP-GlcNAc	-	r

Organism

Organism	UniProt	Comment	Textmining
Campylobacter jejuni	-	NCTC 11168	-

Purification (Commentary)

Purification (Comment)	Organism
MalE-Gne fusion protein overproduced in Escherichia coli	Campylobacter jejuni

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-galactose	-	Campylobacter jejuni	UDP-glucose	-	r
UDP-galactose	the bifunctional enzyme UDP-GlcNAc/Glc 4-epimerase is the sole supplier of the UDP-galactose and UDP-GalNAc required for synthesis of lipooligosaccharide	Campylobacter jejuni	UDP-glucose	-	r
UDP-GalNAc	-	Campylobacter jejuni	UDP-GlcNAc	-	r
UDP-GalNAc	the bifunctional enzyme UDP-GlcNAc/Glc 4-epimerase is the sole supplier of the UDP-galactose and UDP-GalNAc required for synthesis of lipooligosaccharide. The enzyme supplies the UDP-GalNAc required for the synthesis of the capsule	Campylobacter jejuni	UDP-GlcNAc	-	r

Synonyms

Synonyms	Comment	Organism
GNE	-	Campylobacter jejuni
UDP-GlcNAc/Glc 4-epimerase	bifunctional enzyme	Campylobacter jejuni

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
73.95	-	UDP-glucose	37°C	Campylobacter jejuni
82.3	-	UDP-GlcNAc	37°C	Campylobacter jejuni
247.9	-	UDP-GalNAc	37°C	Campylobacter jejuni
290.1	-	UDP-galactose	37°C	Campylobacter jejuni

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Release 2023.1 (January 2023)