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Literature summary for 5.1.3.2 extracted from

  • Prosselkov, P.V.; Gross, W.; Igamberdiev, A.U.; Scharrenberger, C.
    Purification and characterization of UDP-D-galactose 4-epimerase from the red alga Galdieria sulphuraria (1996), Physiol. Plant., 98, 753-758.
No PubMed abstract available
Search for more literature for 5.1.3.2

Inhibitors

Inhibitors Comment Organism Structure
NADH no inhibition Galdieria sulphuraria

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.064
-
 UDPgalactose
-
 Galdieria sulphuraria

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 2 * 42000, SDS-PAGE Galdieria sulphuraria
76000
-
 sucrose density gradient centrifugation Galdieria sulphuraria
83000
-
 gel filtration Galdieria sulphuraria

Organism

Organism UniProt Comment Textmining
Galdieria sulphuraria
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Galdieria sulphuraria

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
42
-
-
 Galdieria sulphuraria

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, stable for 6 months Galdieria sulphuraria

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose
-
 Galdieria sulphuraria UDP-glucose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 42000, SDS-PAGE Galdieria sulphuraria

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
46
-
 no inactivation after several h Galdieria sulphuraria

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Galdieria sulphuraria

pH Range

pH Minimum pH Maximum Comment Organism
6.6 9.2 about 50% of maximal activity at pH 6.6 and 9.2 Galdieria sulphuraria

Cofactor

Cofactor Comment Organism Structure
NAD+ no requirement for external NAD+ Galdieria sulphuraria