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Literature summary for 5.1.3.2 extracted from
- Purification and properties of UDP-galactose 4-epimerase from Bifidobacterium bifidum (1978), Agric. Biol. Chem., 42, 731-737.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| fructose 4-phosphate | stimulates | Bifidobacterium bifidum |  |
| fructose 6-phosphate | stimulates | Bifidobacterium bifidum | |
| Galactose 1-phosphate | stimulates | Bifidobacterium bifidum | |
| glucose 1-phosphate | stimulates | Bifidobacterium bifidum | |
| glucose 6-phosphate | stimulates | Bifidobacterium bifidum | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fructose 1,6-diphosphate | inhibition is enhanced by combination with UMP | Bifidobacterium bifidum | |
| Galactose plus UMP | strong inhibition in the presence of UMP, no inhibition by UMP or sugar alone | Bifidobacterium bifidum | |
| glucose plus UMP | strong inhibition in the presence of UMP, no inhibition by UMP or sugar alone | Bifidobacterium bifidum | |
| HgCl2 | - |
Bifidobacterium bifidum | |
| NEM | - |
Bifidobacterium bifidum | |
| p-chloromercuribenzoate | - |
Bifidobacterium bifidum | |
| UDP | - |
Bifidobacterium bifidum | |
| UTP | slightly | Bifidobacterium bifidum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.53 | - |
UDPgalactose | - |
Bifidobacterium bifidum | |
| 1.4 | - |
UDPglucose | - |
Bifidobacterium bifidum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
2 * 45000, SDS-PAGE | Bifidobacterium bifidum |
| 90000 | - |
gel filtration | Bifidobacterium bifidum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium bifidum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bifidobacterium bifidum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 41.8 | - |
- |
Bifidobacterium bifidum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C or -20°C, 0.2 mg/ml enzyme concentration, 0.01 M potassium phosphate buffer, pH 7.5. About 60% of the initial activity remains for 3 months at 4°C without any protector, and in the presence of 20% glycerol more than 80% of the activity remains | Bifidobacterium bifidum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-galactose | - |
Bifidobacterium bifidum | UDP-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 45000, SDS-PAGE | Bifidobacterium bifidum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Bifidobacterium bifidum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.7 | 7: about 50% of maximal activity, 9.7: about 40% of maximal activity | Bifidobacterium bifidum |
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Release 2023.1 (January 2023)
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