Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | Co2+ is able to activate the enzyme in vitro | Escherichia coli | |
Fe2+ | the enzyme employs a ferrous iron atom as a solvent-exposed cofactor | Escherichia coli | |
Mn2+ | manganese can activate the enzyme in vitro in place of iron converting the enzyme to a form that is unaffected by H2O2 | Escherichia coli | |
additional information | Ca2+, Cd2+, Cu2+, Mg2+, and Ni2+ do not activate the enzyme | Escherichia coli | |
Zn2+ | Zn2+ is able to activate the enzyme in vitro | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Oxidation Stability | Organism |
---|---|
ribulose-5-phosphate 3-epimerase is vulnerable to H2O2 damage in vivo. H2O2 rapidly oxidizes the Fe2+ in a Fenton reaction, the oxidized iron is released immediately, causing a loss of activity. When purified enzyme is loaded with Mn2+, Co2+, or Zn2+, there is no loss in activity when challenged with H2O2 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ribulose 5-phosphate | - |
Escherichia coli | D-Xylulose 5-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ribulose-5-phosphate 3-epimerase | - |
Escherichia coli |
RPE | - |
Escherichia coli |