Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.1.3.1 extracted from

  • Chen, Y.R.; Hartman, F.C.; Lu, T.Y.; Larimer, F.W.
    D-Ribulose-5-phosphate 3-epimerase: cloning and heterologous expression of the spinach gene, and purification and characterization of the recombinant enzyme (1998), Plant Physiol., 118, 199-207.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Spinacia oleracea

General Stability

General Stability Organism
DL-alpha-glycerophosphate or ethanol stabilize the extremely labile recombinant enzyme, but are unable to reverse the spontaneous loss of activity Spinacia oleracea
drastically destabilized by 2-mercaptoethanol Spinacia oleracea
DTT destabilizes Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
DL-alpha-glycerophosphate competitive Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
D-ribulose 5-phosphate 25°C, pH 8.0 Spinacia oleracea

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for a divalent metal ion in catalysis Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
8 * 25000, SDS-PAGE Spinacia oleracea
200000
-
gel filtration Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea Q43157
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11610
-
-
Spinacia oleracea

Storage Stability

Storage Stability Organism
2°C, wether in crude extracts or highly purified, the recombinant enzyme is inherently unstable Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ribulose 5-phosphate
-
Spinacia oleracea D-Xylulose 5-phosphate
-
?

Subunits

Subunits Comment Organism
octamer 8 * 25000, SDS-PAGE Spinacia oleracea

Synonyms

Synonyms Comment Organism
Ru5P epimerase
-
Spinacia oleracea

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.9
-
DL-alpha-glycerophosphate 25°C, pH 8.0 Spinacia oleracea