Cloned (Comment) | Organism |
---|---|
- |
Spinacia oleracea |
General Stability | Organism |
---|---|
DL-alpha-glycerophosphate or ethanol stabilize the extremely labile recombinant enzyme, but are unable to reverse the spontaneous loss of activity | Spinacia oleracea |
drastically destabilized by 2-mercaptoethanol | Spinacia oleracea |
DTT destabilizes | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DL-alpha-glycerophosphate | competitive | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
D-ribulose 5-phosphate | 25°C, pH 8.0 | Spinacia oleracea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for a divalent metal ion in catalysis | Spinacia oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
8 * 25000, SDS-PAGE | Spinacia oleracea |
200000 | - |
gel filtration | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | Q43157 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11610 | - |
- |
Spinacia oleracea |
Storage Stability | Organism |
---|---|
2°C, wether in crude extracts or highly purified, the recombinant enzyme is inherently unstable | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ribulose 5-phosphate | - |
Spinacia oleracea | D-Xylulose 5-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 25000, SDS-PAGE | Spinacia oleracea |
Synonyms | Comment | Organism |
---|---|---|
Ru5P epimerase | - |
Spinacia oleracea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
DL-alpha-glycerophosphate | 25°C, pH 8.0 | Spinacia oleracea |