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Literature summary for 5.1.2.3 extracted from

  • Thieringer, R.; Kunau, W.H.
    The beta-oxidation system in catalase-free microbodies of the filamentous fungus Neurospora crassa. Purification of a multifunctional protein possessing 2-enoyl-CoA hydratase, L-3-hydroxyacyl-CoA dehydrogenase, and 3-hydroxyacyl-CoA epimerase activities (1991), J. Biol. Chem., 266, 13110-13117.
    View publication on PubMed
Search for more literature for 5.1.2.3

Localization

Localization Comment Organism GeneOntology No. Textmining
glycosome exclusively localized in Neurospora crassa 20015
-
glycosome glycosomal microbodies Neurospora crassa 20015
-
microbody exclusively localized in Neurospora crassa 42579
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 trifunctional enzyme which exhibits activities of EC 5.1.2.3, EC 4.2.1.17, and EC 1.1.1.35, MW 365000, gel filtration 1 Neurospora crassa
93000
-
 trifunctional enzyme which exhibits activities of EC 5.1.2.3, EC 4.2.1.17, and EC 1.1.1.35, 4 * 93000, SDS-PAGE, each subunit of the tetrameric protein consists of at least two large domains, the amino-terminal one possessing activities of EC 4.2.1.17 and EC 1.1.1.35 and the carboxy-terminal one bearing activity of EC 5.1.2.3 Neurospora crassa
93000
-
 4 * 93000, trifunctional beta-oxidation protein with activities of EC 1.1.1.35, EC 4.2.1.17 and EC 5.1.2.3, SDS-PAGE Neurospora crassa
365000
-
 trifunctional beta-oxidation protein with activities of EC 1.1.1.35, EC 4.2.1.17 and EC 5.1.2.3, gel filtration Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
trifunctional beta-oxidation protein with activities of EC 1.1.1.35, EC 4.2.1.17 and EC 5.1.2.3 Neurospora crassa
trifunctional enzyme possessing activities of EC 5.1.2.3, EC 1.1.1.35, and EC 4.2.1.17 Neurospora crassa

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Neurospora crassa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Neurospora crassa
52.4
-
 3-hydroxyacyl-CoA epimerase activity of the trifunctional beta-oxidation protein Neurospora crassa

Subunits

Subunits Comment Organism
More trifunctional enzyme which exhibits activities of EC 5.1.2.3, EC 4.2.1.17, and EC 1.1.1.35, 4 * 93000, SDS-PAGE, each subunit of the tetrameric protein consists of at least two large domains, the amino-terminal one possessing activities of EC 4.2.1.17 and EC 1.1.1.35 and the carboxy-terminal one bearing activity of EC 5.1.2.3 Neurospora crassa
tetramer 4 * 93000, trifunctional beta-oxidation protein with activities of EC 1.1.1.35, EC 4.2.1.17 and EC 5.1.2.3, SDS-PAGE Neurospora crassa

Synonyms

Synonyms Comment Organism
TFP trifunctional beta-oxidation protein with activities of EC 1.1.1.35, EC 4.2.1.17 and EC 5.1.2.3 Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Neurospora crassa