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Literature summary for 5.1.1.7 extracted from

  • Pillai, B.; Cherney, M.; Diaper, C.M.; Sutherland, A.; Blanchard, J.S.; Vederas, J.C.; James, M.N.
    Dynamics of catalysis revealed from the crystal structures of mutants of diaminopimelate epimerase (2007), Biochem. Biophys. Res. Commun., 363, 547-553.
    View publication on PubMed

Application

Application Comment Organism
drug development diaminopimelate epimerase catalyzes the stereoinversion of LL-diaminopimelate to meso-diaminopimelate, a precursor of L-lysine and an essential component of the bacterial peptidoglycan. This function is vital to bacteria and the enzyme therefore represents an attractive target for the design of novel anti-bacterials Haemophilus influenzae

Cloned(Commentary)

Cloned (Comment) Organism
DAP epimerase mutants C73S and C217S from Haemophilus influenzae are cloned and purified Haemophilus influenzae

Crystallization (Commentary)

Crystallization (Comment) Organism
comparisons of the mutant structures with the structures of the AziDAP inhibitor-bound form reveal that the enzyme adopts an open conformation in the absence of substrates or inhibitors with the two active site cysteines existing as a thiol–thiolate pair. Substrate binding to the C-terminal domain triggers the closure of the N-terminal domain coupled with tight encapsulation of the ligand, stabilization of the conformation of an active site loop containing Cys73 and expulsion of water molecules with concomitant desolvation of the thiolate base Haemophilus influenzae
crystallization of C73S and C217S mutant diaminopimelate epimerase enzymes of Haemophilus influenzae are obtained by the hanging-drop vapor diffusion method and submitted to X-ray structure analysis Haemophilus influenzae

Protein Variants

Protein Variants Comment Organism
C73S/C217S in order to prevent C73 and C217 of DAP epimerase from oxidation to a disulfide prior to crystallization, DAP epimerase mutants C73S and C217S from Haemophilus influenzae are generated by site-directed mutagenesis Haemophilus influenzae

Inhibitors

Inhibitors Comment Organism Structure
(2-(4-amino-4-carboxybutyl) aziridine-2-carboxylic acid) AziDAP Haemophilus influenzae

Organism

Organism UniProt Comment Textmining
Haemophilus influenzae P44859
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
LL-2,6-Diaminoheptanedioate
-
Haemophilus influenzae meso-Diaminoheptanedioate
-
?

Synonyms

Synonyms Comment Organism
DAP
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Haemophilus influenzae
diaminopimelate epimerase
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Haemophilus influenzae