Application | Comment | Organism |
---|---|---|
drug development | diaminopimelate epimerase catalyzes the stereoinversion of LL-diaminopimelate to meso-diaminopimelate, a precursor of L-lysine and an essential component of the bacterial peptidoglycan. This function is vital to bacteria and the enzyme therefore represents an attractive target for the design of novel anti-bacterials | Haemophilus influenzae |
Cloned (Comment) | Organism |
---|---|
DAP epimerase mutants C73S and C217S from Haemophilus influenzae are cloned and purified | Haemophilus influenzae |
Crystallization (Comment) | Organism |
---|---|
comparisons of the mutant structures with the structures of the AziDAP inhibitor-bound form reveal that the enzyme adopts an open conformation in the absence of substrates or inhibitors with the two active site cysteines existing as a thiolthiolate pair. Substrate binding to the C-terminal domain triggers the closure of the N-terminal domain coupled with tight encapsulation of the ligand, stabilization of the conformation of an active site loop containing Cys73 and expulsion of water molecules with concomitant desolvation of the thiolate base | Haemophilus influenzae |
crystallization of C73S and C217S mutant diaminopimelate epimerase enzymes of Haemophilus influenzae are obtained by the hanging-drop vapor diffusion method and submitted to X-ray structure analysis | Haemophilus influenzae |
Protein Variants | Comment | Organism |
---|---|---|
C73S/C217S | in order to prevent C73 and C217 of DAP epimerase from oxidation to a disulfide prior to crystallization, DAP epimerase mutants C73S and C217S from Haemophilus influenzae are generated by site-directed mutagenesis | Haemophilus influenzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2-(4-amino-4-carboxybutyl) aziridine-2-carboxylic acid) | AziDAP | Haemophilus influenzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | P44859 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
LL-2,6-Diaminoheptanedioate | - |
Haemophilus influenzae | meso-Diaminoheptanedioate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DAP | - |
Haemophilus influenzae |
diaminopimelate epimerase | - |
Haemophilus influenzae |