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Literature summary for 5.1.1.7 extracted from
- Purification and properties of diaminopimelic acid epimerase from Escherichia coli (1984), J. Biol. Chem., 259, 8907-8914.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | one, but not both, of the proton acceptor sites is a thiol | Escherichia coli | |
| additional information | no pyridoxal phosphate requirement | Escherichia coli |
General Stability
| General Stability | Organism |
|---|---|
| air oxidation, in absence of reducing thiols, is slower at pH 7 than at pH 8 | Escherichia coli |
| denatured on freezing | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hydroxylamine | no inhibition | Escherichia coli |  |
| iodoacetamide | half-life for inactivation with 0.25 mM iodoacetamide is 9.6 min | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
LL-diaminoheptanedioate | - |
Escherichia coli | |
| 0.36 | - |
meso-diaminoheptanedioate | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
1 * 34000, SDS-PAGE | Escherichia coli |
| 45400 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, best storage temperature | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| LL-2,6-Diaminoheptanedioate | - |
Escherichia coli | meso-Diaminoheptanedioate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 34000, SDS-PAGE | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 67 | - |
meso-diaminoheptanedioate | - |
Escherichia coli | |
| 84 | - |
LL-diaminoheptanedioate | - |
Escherichia coli | |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
irreversible loss of activity at pH 6 and below. Partially reactivated by thiols | Escherichia coli |
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