Application | Comment | Organism |
---|---|---|
synthesis | production of D-glutamate from L-glutamate with glutamate racemase and L-glutamate oxidase from Streptomyces sp. X119-6. Both enzymes are highly stabilized by immobilization | Bacillus subtilis |
General Stability | Organism |
---|---|
stabilized by immobilization to Chitopearl 2505 or Chitopearl 2605. The enzyme remains active at 4°C for 30 days, remaining activity is 32% | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
IFO 3336 | - |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
soluble and immobilized enzyme | Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | 20°C: about 65% of maximal activity, 70°C: about 30% of maximal activity, soluble enzyme | Bacillus subtilis |
20 | 70 | more than 80% of maximal activity is observed between 20°C and 70°C, immobilized enzyme | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
pH 7, 60 min, about 25% loss of activity of soluble and immobilized enzyme | Bacillus subtilis |
80 | - |
pH 7, 60 min, about 20% loss of activity of immobilized enzyme, about 70% loss of activity of soluble enzyme | Bacillus subtilis |
90 | - |
pH 7, 60 min, 50% loss of activity of immobilized enzyme, about 75% loss of activity of soluble enzyme | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
soluble and immobilized enzyme | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 10 | pH 2.0: about 40% of maximal activity of soluble enzyme, about 60% of maximal activity of immobilized enzyme, pH 10.0: about 80% of maximal activity of immobilized enzyme, about 40% of maximal activity of soluble enzyme | Bacillus subtilis |