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Literature summary for 5.1.1.3 extracted from
- Properties of glutamate racemase from Bacillus subtilis IFO 3336 producing poly-gamma-glutamate (1998), J. Biochem., 123, 1156-1163.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | cofactor-independent enzyme | Bacillus subtilis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli JM109 | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-nitro-5-thiocyanatobenzoate | 0.2 mM | Bacillus subtilis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | - |
D-glutamate | pH 8.0, 37°C | Bacillus subtilis | |
| 10 | - |
D-Homocysteinesulfinate | pH 8.0, 37°C | Bacillus subtilis | |
| 50 | - |
L-glutamate | pH 8.0, 37°C | Bacillus subtilis | |
| 200 | - |
L-Homocysteinesulfinate | pH 8.0, 37°C | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29866 | - |
1 * 29866, calculation from nucleotide sequence | Bacillus subtilis |
| 30000 | - |
- |
Bacillus subtilis |
| 30000 | - |
gel filtration | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Glu | Bacillus subtilis | glutamate racemase is mainly concerned in D-Glu synthesis for poly-gamma-glutamate production | ? | - |
? | |
| L-Glu | Bacillus subtilis CU741 | glutamate racemase is mainly concerned in D-Glu synthesis for poly-gamma-glutamate production | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis | - |
CU741 | - |
| Bacillus subtilis | - |
IFO 3336 | - |
| Bacillus subtilis | P94556 | IFO 3336 | - |
| Bacillus subtilis CU741 | - |
CU741 | - |
| Pediococcus pentosaceus | - |
IFO 3182 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | highest activity in the early stationary phase of growth | Bacillus subtilis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 42 | - |
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Glu | - |
Bacillus subtilis | L-Glu | - |
? | |
| D-Glu | - |
Bacillus subtilis CU741 | L-Glu | - |
? | |
| L-Glu | glutamate racemase is mainly concerned in D-Glu synthesis for poly-gamma-glutamate production | Bacillus subtilis | ? | - |
? | |
| L-Glu | glutamate racemase is mainly concerned in D-Glu synthesis for poly-gamma-glutamate production | Bacillus subtilis CU741 | ? | - |
? | |
| L-glutamate | - |
Bacillus subtilis | D-glutamate | - |
? | |
| L-Homocysteinesulfinate | - |
Bacillus subtilis | D-Homocysteinesulfinate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 30000, SDS-PAGE | Bacillus subtilis |
| monomer | 1 * 30000 | Bacillus subtilis |
| monomer | 1 * 29866, calculation from nucleotide sequence | Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
fully stable up to | Bacillus subtilis |
| 50 | - |
pH 8.0, 0.1 M Tris-HCl buffer containing 10% glycerol, retains 90% of its activity after 10 min | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | enzyme shows significant activity in the range pH 7.0-10.0 | Bacillus subtilis |
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