Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pediococcus pentosaceus |
General Stability | Organism |
---|---|
irreversible loss of activity on storage at DL-Glu and a reducing thiol such as 2-mercaptoethanol. Inactivation may be due to air oxidation of a free thiol residue | Pediococcus pentosaceus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Nitro-5-thiocyanobenzoate | - |
Pediococcus pentosaceus | |
5,5'-dithiobis(2-nitrobenzoate) | - |
Pediococcus pentosaceus | |
hydroxylamine | no inactivation | Pediococcus pentosaceus | |
additional information | no inactivation by carbonyl reagents such as hydroxylamine and NaBH4 | Pediococcus pentosaceus | |
p-chloromercuribenzoate | - |
Pediococcus pentosaceus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
D-Glu | - |
Pediococcus pentosaceus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
HPLC gel filtration | Pediococcus pentosaceus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pediococcus pentosaceus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pediococcus pentosaceus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26 | - |
- |
Pediococcus pentosaceus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glu | - |
Pediococcus pentosaceus | L-Glu | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE | Pediococcus pentosaceus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pediococcus pentosaceus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pediococcus pentosaceus |