Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Bifidobacterium bifidum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | 1 mM, complete inhibition, pH 7.0, 30°C, 30 min | Bifidobacterium bifidum | |
alpha-methyl-DL-aspartic acid | 10 mM, 12% inhibition | Bifidobacterium bifidum | |
HgCl2 | 0.1 M, complete inhibition, pH 7.0, 30°C, 30 min | Bifidobacterium bifidum | |
NEM | 1 mM, complete inhibition, pH 7.0, 30°C, 30 min | Bifidobacterium bifidum | |
PCMB | 0.1 M, complete inhibition, pH 7.0, 30°C, 30 min | Bifidobacterium bifidum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.94 | - |
D-Asp | - |
Bifidobacterium bifidum | |
13.4 | - |
L-Asp | - |
Bifidobacterium bifidum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
gel filtration | Bifidobacterium bifidum |
30000 | - |
1 * 30000, SDS-PAGE | Bifidobacterium bifidum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium bifidum | - |
NBRC 14252 | - |
Bifidobacterium bifidum NBRC 14252 | - |
NBRC 14252 | - |
Purification (Comment) | Organism |
---|---|
- |
Bifidobacterium bifidum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.75 | - |
- |
Bifidobacterium bifidum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Asp | enzyme exclusively acts on L-Asp and D-Asp | Bifidobacterium bifidum | D-Asp | - |
r | |
L-Asp | enzyme exclusively acts on L-Asp and D-Asp | Bifidobacterium bifidum NBRC 14252 | D-Asp | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, SDS-PAGE | Bifidobacterium bifidum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bifidobacterium bifidum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
30 min, stable up to 30°C in 0.1 M Tes buffer, pH 6.5 containing 4 mM dithiothreitol and 1 mM EDTA | Bifidobacterium bifidum |
60 | - |
30 min, stable up to 60°C in presence of 40 mM D-Asp | Bifidobacterium bifidum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Bifidobacterium bifidum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | 30°C, 30 min, stable | Bifidobacterium bifidum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme does not require a cofactor | Bifidobacterium bifidum |