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Literature summary for 5.1.1.13 extracted from

  • Yamashita, T.; Ashiuchi, M.; Ohnishi, K.; Kato, S.; Nagata, S.; Misono, H.
    Molecular identification of monomeric aspartate racemase from Bifidobacterium bifidum (2004), Eur. J. Biochem., 271, 4798-4803.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Bifidobacterium bifidum

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) 1 mM, complete inhibition, pH 7.0, 30°C, 30 min Bifidobacterium bifidum
alpha-methyl-DL-aspartic acid 10 mM, 12% inhibition Bifidobacterium bifidum
HgCl2 0.1 M, complete inhibition, pH 7.0, 30°C, 30 min Bifidobacterium bifidum
NEM 1 mM, complete inhibition, pH 7.0, 30°C, 30 min Bifidobacterium bifidum
PCMB 0.1 M, complete inhibition, pH 7.0, 30°C, 30 min Bifidobacterium bifidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.94
-
D-Asp
-
Bifidobacterium bifidum
13.4
-
L-Asp
-
Bifidobacterium bifidum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
gel filtration Bifidobacterium bifidum
30000
-
1 * 30000, SDS-PAGE Bifidobacterium bifidum

Organism

Organism UniProt Comment Textmining
Bifidobacterium bifidum
-
NBRC 14252
-
Bifidobacterium bifidum NBRC 14252
-
NBRC 14252
-

Purification (Commentary)

Purification (Comment) Organism
-
Bifidobacterium bifidum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.75
-
-
Bifidobacterium bifidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Asp enzyme exclusively acts on L-Asp and D-Asp Bifidobacterium bifidum D-Asp
-
r
L-Asp enzyme exclusively acts on L-Asp and D-Asp Bifidobacterium bifidum NBRC 14252 D-Asp
-
r

Subunits

Subunits Comment Organism
monomer 1 * 30000, SDS-PAGE Bifidobacterium bifidum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Bifidobacterium bifidum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
30 min, stable up to 30°C in 0.1 M Tes buffer, pH 6.5 containing 4 mM dithiothreitol and 1 mM EDTA Bifidobacterium bifidum
60
-
30 min, stable up to 60°C in presence of 40 mM D-Asp Bifidobacterium bifidum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Bifidobacterium bifidum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7 30°C, 30 min, stable Bifidobacterium bifidum

Cofactor

Cofactor Comment Organism Structure
additional information enzyme does not require a cofactor Bifidobacterium bifidum