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Literature summary for 4.99.1.4 extracted from

  • Stroupe, M.E.; Leech, H.K.; Daniels, D.S.; Warren, M.J.; Getzoff, E.D.
    CysG structure reveals tetrapyrrole-binding features and novel regulation of siroheme biosynthesis (2003), Nat. Struct. Biol., 10, 1064-1073.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Salmonella enterica

Crystallization (Commentary)

Crystallization (Comment) Organism
X-ray crystal structure of CysG Salmonella enterica

Protein Variants

Protein Variants Comment Organism
S128A mutant has higher cobalt chelatase activity than wild-type CysG with sirohydrochlorin as substrate Salmonella enterica
S128D mutant has lower cobalt chelatase activity than wild-type CysG with sirohydrochlorin as substrate Salmonella enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sirohydrochlorin + Fe2+ Salmonella enterica CysG, siroheme biosynthesis siroheme + 2 H+ sulfur metabolism depends on siroheme ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein CysG, phosphorylation of Ser-128, plays a regulatory role Salmonella enterica

Purification (Commentary)

Purification (Comment) Organism
-
Salmonella enterica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0138
-
S128D mutant CysG, cobalt chelation of sirohydrochlorin Salmonella enterica
0.0656
-
wild-type CysG, cobalt chelation of sirohydrochlorin Salmonella enterica
0.243
-
S128A mutant CysG, cobalt chelation of sirohydrochlorin Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sirohydrochlorin + Co2+
-
Salmonella enterica cobalt-sirohydrochlorin + 2 H+
-
?
sirohydrochlorin + Fe2+ CysG structure, the multifunctional siroheme synthase CysG synthesizes siroheme from uroporphyrinogen III, CysG contains two structurally independent modules: a bismethyltransferase and a dual-function dehydrogenase-chelatase Salmonella enterica siroheme + 2 H+
-
?
sirohydrochlorin + Fe2+ CysG, siroheme biosynthesis Salmonella enterica siroheme + 2 H+ sulfur metabolism depends on siroheme ?

Subunits

Subunits Comment Organism
homodimer
-
Salmonella enterica

Synonyms

Synonyms Comment Organism
CysG Salmonella enterica Salmonella enterica