Literature summary for 4.99.1.3 extracted from

Lundqvist, J.; Elmlund, D.; Heldt, D.; Deery, E.; Soederberg, C.A.; Hansson, M.; Warren, M.; Al-Karadaghi, S.
The AAA(+) motor complex of subunits CobS and CobT of cobaltochelatase visualized by single particle electron microscopy (2009), J. Struct. Biol., 167, 227-234.

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Cloned(Commentary)

Cloned (Comment)	Organism
genes cobN, cobS and cobT, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3)	Brucella melitensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37000	-	x * 137000, subunit CobN, + x * 37000, subunit CobS, + x * 71000, subunit CobT	Brucella melitensis
71000	-	x * 137000, subunit CobN, + x * 37000, subunit CobS, + x * 71000, subunit CobT	Brucella melitensis
137000	-	x * 137000, subunit CobN, + x * 37000, subunit CobS, + x * 71000, subunit CobT	Brucella melitensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sirohydrochlorin + Co2+	Brucella melitensis	involved in the formation of a octahedrally co-ordinated cobalt ion. In the oxygen-dependent cobalamin, i.e. vitamin B12, biosynthetic pathway, cobalt is inserted into a ring-contracted tetrapyrrole called hydrogenobyrinic acid a,c-diamide, HBAD, by a cobaltochelatase that is constituted by three subunits, CobN, CobS and CobT	cobalt-sirohydrochlorin + 2 H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Brucella melitensis	-	genes cobN, cobS and cobT encoding the tree subunits	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sirohydrochlorin + Co2+	involved in the formation of a octahedrally co-ordinated cobalt ion. In the oxygen-dependent cobalamin, i.e. vitamin B12, biosynthetic pathway, cobalt is inserted into a ring-contracted tetrapyrrole called hydrogenobyrinic acid a,c-diamide, HBAD, by a cobaltochelatase that is constituted by three subunits, CobN, CobS and CobT	Brucella melitensis	cobalt-sirohydrochlorin + 2 H+	-	?

Subunits

Subunits	Comment	Organism
More	the molecules are arranged in a two-tiered ring structure with the six subunits in each ring organized as a trimer of dimers, subunits CobS and CobT form a chaperone-like complex. Homology modelling of subunit CobS, overview	Brucella melitensis
oligomer	x * 137000, subunit CobN, + x * 37000, subunit CobS, + x * 71000, subunit CobT	Brucella melitensis

Synonyms

Synonyms	Comment	Organism
cobaltochelatase	-	Brucella melitensis
More	the enzyme belongs to the AAA+ superfamily of proteins	Brucella melitensis

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Release 2023.1 (January 2023)