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Literature summary for 4.99.1.3 extracted from
- A story of chelatase evolution: Identification and characterisation of a small 13-15 kDa 'ancestral' cobaltochelatase (CbiXS) in the Archaea (2003), J. Biol. Chem., 278, 22388-22395.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overproduced in Escherichia coli | Methanosarcina barkeri |
| overproduced in Escherichia coli | Methanobacter thermoautotrophicum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | insertion of cobalt into sirohydrochlorin | Methanosarcina barkeri |  |
| Co2+ | insertion of cobalt into sirohydrochlorin | Methanobacter thermoautotrophicum | |
| Fe2+ | able to chelate | Methanosarcina barkeri | |
| Fe2+ | able to chelate | Methanobacter thermoautotrophicum | |
| Ni2+ | able to chelate nickel | Methanobacter thermoautotrophicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 14000 | - |
gene-predicted molecular mass | Methanosarcina barkeri |
| 14000 | - |
gene-predicted molecular mass | Methanobacter thermoautotrophicum |
| 15000 | - |
SDS-PAGE | Methanosarcina barkeri |
| 17000 | - |
SDS-PAGE | Methanobacter thermoautotrophicum |
| 40000 | - |
gel filtration | Methanosarcina barkeri |
| 40000 | - |
gel filtration | Methanobacter thermoautotrophicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sirohydrochlorin + Co2+ | Methanosarcina barkeri | cobalamin biosynthetic pathway | cobalt-sirohydrochlorin + H+ | - |
? | |
| sirohydrochlorin + Co2+ | Methanobacter thermoautotrophicum | cobalamin biosynthetic pathway | cobalt-sirohydrochlorin + H+ | - |
? | |
| sirohydrochlorin + Co2+ | Methanosarcina barkeri Fusaro / DSM 804 | cobalamin biosynthetic pathway | cobalt-sirohydrochlorin + H+ | - |
? | |
| sirohydrochlorin + Fe2+ | Methanosarcina barkeri | CbiXS can act as a ferrochelatase in the biosynthesis of siroheme in vivo | siroheme + H+ | - |
? | |
| sirohydrochlorin + Fe2+ | Methanobacter thermoautotrophicum | CbiXS can act as a ferrochelatase in the biosynthesis of siroheme in vivo | siroheme + H+ | - |
? | |
| sirohydrochlorin + Fe2+ | Methanosarcina barkeri Fusaro / DSM 804 | CbiXS can act as a ferrochelatase in the biosynthesis of siroheme in vivo | siroheme + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanobacter thermoautotrophicum | - |
- |
- |
| Methanosarcina barkeri | - |
- |
- |
| Methanosarcina barkeri Fusaro / DSM 804 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanosarcina barkeri |
- |
Methanobacter thermoautotrophicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.018 | - |
- |
Methanobacter thermoautotrophicum |
| 0.06 | - |
specific activity to chelate nickel | Methanobacter thermoautotrophicum |
| 0.122 | - |
- |
Methanosarcina barkeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | precorrin-2 is no substrate | Methanosarcina barkeri | ? | - |
? | |
| additional information | precorrin-2 is no substrate | Methanobacter thermoautotrophicum | ? | - |
? | |
| additional information | precorrin-2 is no substrate | Methanosarcina barkeri Fusaro / DSM 804 | ? | - |
? | |
| sirohydrochlorin + Co2+ | - |
Methanosarcina barkeri | cobalt-sirohydrochlorin + H+ | - |
? | |
| sirohydrochlorin + Co2+ | - |
Methanobacter thermoautotrophicum | cobalt-sirohydrochlorin + H+ | - |
? | |
| sirohydrochlorin + Co2+ | cobalamin biosynthetic pathway | Methanosarcina barkeri | cobalt-sirohydrochlorin + H+ | - |
? | |
| sirohydrochlorin + Co2+ | cobalamin biosynthetic pathway | Methanobacter thermoautotrophicum | cobalt-sirohydrochlorin + H+ | - |
? | |
| sirohydrochlorin + Co2+ | - |
Methanosarcina barkeri Fusaro / DSM 804 | cobalt-sirohydrochlorin + H+ | - |
? | |
| sirohydrochlorin + Co2+ | cobalamin biosynthetic pathway | Methanosarcina barkeri Fusaro / DSM 804 | cobalt-sirohydrochlorin + H+ | - |
? | |
| sirohydrochlorin + Fe2+ | CbiXS can act as a ferrochelatase in the biosynthesis of siroheme in vivo | Methanosarcina barkeri | siroheme + H+ | - |
? | |
| sirohydrochlorin + Fe2+ | CbiXS can act as a ferrochelatase in the biosynthesis of siroheme in vivo | Methanobacter thermoautotrophicum | siroheme + H+ | - |
? | |
| sirohydrochlorin + Fe2+ | CbiXS can act as a ferrochelatase in the biosynthesis of siroheme in vivo | Methanosarcina barkeri Fusaro / DSM 804 | siroheme + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| archaeal cobaltochelatase | - |
Methanosarcina barkeri |
| archaeal cobaltochelatase | - |
Methanobacter thermoautotrophicum |
| CbiX | - |
Methanosarcina barkeri |
| CbiX | - |
Methanobacter thermoautotrophicum |
| CbiXS | - |
Methanosarcina barkeri |
| CbiXS | - |
Methanobacter thermoautotrophicum |
| cobaltochelatase | - |
Methanosarcina barkeri |
| cobaltochelatase | - |
Methanobacter thermoautotrophicum |
| sirohydrochlorin cobaltochelatase | - |
Methanosarcina barkeri |
| sirohydrochlorin cobaltochelatase | - |
Methanobacter thermoautotrophicum |
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