Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type and mutant MerBs. MerB, C96S MerB, C159S MerB, and C160S MerB, in the free and mercury-bound form, by vapor diffusion at 23°C and pH 5.5 using either a 1:1 or 1:2 mixture of protein solution, containing 8 mg/ml initial protein concentration, and precipitant buffer, respectively, that is equilibrated against a reservoir of precipitant buffer , X-ray diffraction structure determination and analysis at 1.6-1.8 A resolution | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C159S | site-directed mutagenesis, inactive mutant | Escherichia coli |
C160S | site-directed mutagenesis, mutant enzyme with moderately reduced activity | Escherichia coli |
C96S | site-directed mutagenesis, inactive mutant | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P77072 | gene merB encoded on plasmid R831b | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | mechanism for the cleavage of the carbon-mercury bond and the formation of the MerB-Hg complex, modelling, overview. Hg2+ binding structure of wild-type and mutant enzymes, overview | Escherichia coli | ? | - |
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Synonyms | Comment | Organism |
---|---|---|
merB | - |
Escherichia coli |