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Literature summary for 4.99.1.2 extracted from

  • Tezuka, T.; Tonomura, K.
    Purification and properties of an enzyme catalyzing the splitting of carbon-mercury linkages from mercury-resistant Pseudomonas K-62 strain. I. Splitting enzyme 1 (1976), J. Biochem., 80, 79-87.
    View publication on PubMed

General Stability

General Stability Organism
inactivated by freezing Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.053
-
p-chloromercuribenzoic acid
-
Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
K-62
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Pseudomonas sp.

Storage Stability

Storage Stability Organism
stable for at least 3 months, pH 7.5, 5% glycerol, 2°C Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethylmercuric chloride + H+
-
Pseudomonas sp. C2H6 + Hg2+ + Cl-
-
?
methylmercuric chloride + H+
-
Pseudomonas sp. CH4 + Hg2+ + Cl-
-
?
additional information
-
Pseudomonas sp. ?
-
?
p-chloromercuribenzoate
-
Pseudomonas sp. Hg2+ + p-hydroxybenzoate
-
?
phenylmercuric acetate + H+
-
Pseudomonas sp. benzene + Hg+ + Cl-
-
?
RHg+ + H+
-
Pseudomonas sp. RH + Hg2+
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Pseudomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60
-
Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9
-
Pseudomonas sp.