Cloned (Comment) | Organism |
---|---|
a 339 bp core fragment and full-length DNA of MECPS amplified and subcloned into pGEM T-easy vector. Escherichia coli harboring both of the plasmids, pAC-BETA and pTrc or pTrcTmMECPS or pTrcAtMECPS | Taxus x media |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | active site including a Zn2+ ligand (Asp92-His94-His126) | Taxus x media |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26100 | - |
sequence analysis | Taxus x media |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Taxus x media | D3G6G3 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bark | tender bark, highest level | Taxus x media | - |
leaf | highest level | Taxus x media | - |
root | middle level | Taxus x media | - |
stem | lowestlevel | Taxus x media | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | active site including CDP-binding sites (Asp140Gly142 and Leu184Leu189Ser190Lys216Thr217Glu219). Owns a 60 amino-acid plastidial transit peptide at its N-terminus | Taxus x media | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26100, calculated from sequence | Taxus x media |
Synonyms | Comment | Organism |
---|---|---|
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase | - |
Taxus x media |
MECPS | - |
Taxus x media |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Taxus x media | sequence analysis | - |
8.22 |
Organism | Comment | Expression |
---|---|---|
Taxus x media | higher expression levels in tender barks and leaves than that in roots and stems | up |
General Information | Comment | Organism |
---|---|---|
physiological function | is involved in the taxol precursor biosynthesis. Escherichia coli harboring both of the plasmids, pAC-BETA and pTrc or pTrcTmMECPS or pTrcAtMECPS can grow well, while Escherichia coli without exogenous plasmid or harboring either of the plasmids can not grow | Taxus x media |