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Literature summary for 4.4.1.5 extracted from

  • Hossain, M.A.; Fujita, M.
    Purification of glyoxalase I from onion bulbs and molecular cloning of its cDNA (2009), Biosci. Biotechnol. Biochem., 73, 2007-2013.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli XL1-Blue cells Allium cepa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
calculated from amino acid sequence Allium cepa
25000
-
SDS-PAGE Allium cepa

Organism

Organism UniProt Comment Textmining
Allium cepa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-cellulose column chromatography, hydroxyapatite column chromatography, and S-hexylglutathione-agarose column chromatography Allium cepa

Source Tissue

Source Tissue Comment Organism Textmining
bulb the highest glyoxalase I concentration is found in mature bulbs Allium cepa
-
leaf
-
Allium cepa
-
root
-
Allium cepa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.94
-
crude extract, at 22°C Allium cepa
356
-
after 184fold purification, at 22°C Allium cepa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methylglyoxal + glutathione
-
Allium cepa (R)-S-lactoylglutathione
-
?

Synonyms

Synonyms Comment Organism
glyoxalase I
-
Allium cepa

Cofactor

Cofactor Comment Organism Structure
glutathione
-
Allium cepa

pI Value

Organism Comment pI Value Maximum pI Value
Allium cepa calculated from amino acid sequence
-
5.2

Expression

Organism Comment Expression
Allium cepa glyoxlase I activity decreases in heavy metal stress (exposure to 0.5 mM CdCl2) down
Allium cepa low temperature (4°C) stress shows the highest induction (2.6fold) of glyoxalase I activity, followed by salinity (exposure to 400 mM NaCl) and drought (exposure to 10% (v/v) PEG) stress for 7 days up

General Information

General Information Comment Organism
physiological function GLOI plays an important role in the plant abiotic stress response Allium cepa