Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
methylglyoxal | 10 mM, enhanced tolerance to toxic stress in transgenic Vigna mungo | Brassica juncea | |
NaCl | the transgenic and the untransformed plants are exposed to 100 mM NaCl. The transgenic plants survived whereas the untransformed control plants fail to survive | Brassica juncea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
Western blot analysis | Brassica juncea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylglyoxal + glutathione | Brassica juncea | glyoxalase I is a member of the metallogltathione transferase superfamily and plays a critical role in detoxification of the cytotoxic methylglyoxal to S-D-lactoylglutathione via 1,2-hydrogen transfer | S-((R)-lactoyl)glutathione | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica juncea | - |
gene gly I coding for glyoxalase I transformed into Vigna mungo L. Hepper using Agrobacterium tumefaciens, controlled by a novel constitutive Cestrum yellow leaf curling viral promotor | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | young leaves of the transgenic plant Vigna mungo, transformed with gly1 from Brassica juncae using a transformation system via Agrobacterium tumefaciens, driven by a constitutive Cestrum yellow leaf curling viral promotor | Brassica juncea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the GLO1 activity of the transformed Vigna mungo plants is higher than in the untransformed control plants | Brassica juncea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylglyoxal + glutathione | glyoxalase I is a member of the metallogltathione transferase superfamily and plays a critical role in detoxification of the cytotoxic methylglyoxal to S-D-lactoylglutathione via 1,2-hydrogen transfer | Brassica juncea | S-((R)-lactoyl)glutathione | - |
? | |
methylglyoxal + glutathione | non-enzymatic formation of hemithioacetal from methylglyoxal and reduced glutathione | Brassica juncea | S-((R)-lactoyl)glutathione | enzyme activity is measured spectrophotometrically as a function of thioester formation, S-((R)-lactoyl)glutathione, by measuring the rate of change of absorbance at 240 nm | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, Western blot analysis | Brassica juncea |
Synonyms | Comment | Organism |
---|---|---|
Gly I | - |
Brassica juncea |
glyoxalase I | - |
Brassica juncea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme assay at room temperature | Brassica juncea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Brassica juncea |