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Literature summary for 4.4.1.5 extracted from

  • Akoachere, M.; Iozef, R.; Rahlfs, S.; Deponte, M.; Mannervik, B.; Creighton, D.J.; Schirmer, H.; Becker, K.
    Characterization of the glyoxalases of the malarial parasite Plasmodium falciparum and comparison with their human counterparts (2005), Biol. Chem., 386, 41-52.
    View publication on PubMed
Search for more literature for 4.4.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens
expression in Escherichia coli Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
S-(N-hydroxy-N-bromophenylcarbamoyl)gluthatione IC50 0.06 microM Homo sapiens
S-(N-hydroxy-N-bromophenylcarbamoyl)gluthatione IC50 0.06 microM Plasmodium falciparum
S-(N-hydroxy-N-chlorophenylcarbamoyl)gluthatione IC50 0.09 microM Homo sapiens
S-(N-hydroxy-N-chlorophenylcarbamoyl)gluthatione IC50 0.11 microM Plasmodium falciparum
S-(N-hydroxy-N-phenylcarbamoyl)gluthatione IC50 2.5 microM Homo sapiens
S-(N-hydroxy-N-phenylcarbamoyl)gluthatione IC50 10 microM Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
 (R)-S-lactoylglutathione 30°C, pH 7.0 Plasmodium falciparum
0.09
-
 (R)-S-lactoylglutathione 30°C, pH 7.0 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Plasmodium falciparum 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ addition of ZnSO4 during the overexpression results in increased catalytic activity and a decreased Km value Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35840
-
 calculated from amino acid sequence Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Plasmodium falciparum
-
 active cytosolic glyoxalase 1, inactive glyoxalase 1-like protein
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens
-
 Plasmodium falciparum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
110
-
 30°C, pH 7.0 Homo sapiens
150
-
 30°C, pH 7.0 Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-S-lactoylglutathione
-
 Homo sapiens glutathione + methylglyoxal
-
 ?
(R)-S-lactoylglutathione
-
 Plasmodium falciparum glutathione + methylglyoxal
-
 r

Synonyms

Synonyms Comment Organism
Glo1
-
 Homo sapiens
Glo1
-
 Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00003
-
 S-(N-hydroxy-N-bromophenylcarbamoyl)gluthatione
-
 Homo sapiens
0.00005
-
 S-(N-hydroxy-N-chlorophenylcarbamoyl)gluthatione
-
 Homo sapiens
0.00006
-
 S-(N-hydroxy-N-bromophenylcarbamoyl)gluthatione
-
 Plasmodium falciparum
0.00008
-
 S-(N-hydroxy-N-chlorophenylcarbamoyl)gluthatione
-
 Plasmodium falciparum

pI Value

Organism Comment pI Value Maximum pI Value
Plasmodium falciparum calculated
-
 8.59

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00006
-
 IC50 0.06 microM Homo sapiens S-(N-hydroxy-N-bromophenylcarbamoyl)gluthatione
0.00006
-
 IC50 0.06 microM Plasmodium falciparum S-(N-hydroxy-N-bromophenylcarbamoyl)gluthatione
0.00009
-
 IC50 0.09 microM Homo sapiens S-(N-hydroxy-N-chlorophenylcarbamoyl)gluthatione
0.00011
-
 IC50 0.11 microM Plasmodium falciparum S-(N-hydroxy-N-chlorophenylcarbamoyl)gluthatione
0.0025
-
 IC50 2.5 microM Homo sapiens S-(N-hydroxy-N-phenylcarbamoyl)gluthatione
0.01
-
 IC50 10 microM Plasmodium falciparum S-(N-hydroxy-N-phenylcarbamoyl)gluthatione