Literature summary for 4.4.1.5 extracted from

Lupidi, G.; Venardi, G.; Bollettini, M.; Marmocchi, F.; Rotilio, G.
Purification and partial characterization of glyoxalase I from bovine brain (2001), Prep. Biochem. Biotechnol., 31, 305-316.

Search for more literature for 4.4.1.5

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	2 mM, approx. 50% inactivation after 10 min, almost complete inhibition after 180 min	Bos taurus
2,6-pyridindicarboxylic acid	2 mM, approx. 50% inactivation after 10 min, almost complete inhibition after 180 min	Bos taurus
EDTA	0.1 mM, approx. 50% inactivation after 50 min, approx. 80% inhibition after 250 min	Bos taurus
S-bromobenzylglutathione	-	Bos taurus
S-bromobenzylglutathione	-	Saccharomyces cerevisiae
S-p-nitrobenzylglutathione	-	Bos taurus
S-p-nitrobenzylglutathione	-	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.29	-	glutathione-methylglyoxal hemithioacetal	-	Bos taurus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	incubation of the Zn2+ depleted apoenzyme with Mg2+ restores 50% of the enzyme activity	Bos taurus
Zn2+	1 mol Zn2+ per subunit	Bos taurus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
22000	-	2 * 22000, SDS-PAGE	Bos taurus
44000	-	gel filtration	Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
glutathione-methylglyoxal hemithioacetal	Saccharomyces cerevisiae	glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione	(R)-S-lactoylglutathione	-	?
glutathione-methylglyoxal hemithioacetal	Bos taurus	glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione	(R)-S-lactoylglutathione	-	?

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharomyces cerevisiae
60°C, DEAE-52 cellulose, S-hexylglutathione Sepharose 6 B affinity, gel filtraton	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
425	-	-	Bos taurus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glutathione-methylglyoxal hemithioacetal	glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione	Saccharomyces cerevisiae	(R)-S-lactoylglutathione	-	?
glutathione-methylglyoxal hemithioacetal	glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione	Bos taurus	(R)-S-lactoylglutathione	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 22000, SDS-PAGE	Bos taurus

Synonyms

Synonyms	Comment	Organism
glyoxalase I	-	Saccharomyces cerevisiae
glyoxalase I	-	Bos taurus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0057	-	S-bromobenzylglutathione	-	Saccharomyces cerevisiae
0.021	-	S-bromobenzylglutathione	-	Bos taurus
0.032	-	S-p-nitrobenzylglutathione	-	Bos taurus
0.083	-	S-p-nitrobenzylglutathione	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)