Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
E163Q | 58% of wild-type kcat | Saccharomyces cerevisiae |
E163Q/E318Q | 0.02% of wild-type kcat | Saccharomyces cerevisiae |
E318Q | 16% of wild-type kcat | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-hexylglutathione | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
glutathione-methylglyoxal hemithioacetal | 30°C, pH 7.0 | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | at least one iron per enzyme molecule, the second metal is zinc or manganese, apparantly depending on growth conditions | Saccharomyces cerevisiae | |
Mn2+ | can replace Zn2+ | Saccharomyces cerevisiae | |
Zn2+ | - |
Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione-methylglyoxal hemithioacetal | Saccharomyces cerevisiae | glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione | (R)-S-lactoylglutathione | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant glyoxalase I | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione-methylglyoxal hemithioacetal | glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione | Saccharomyces cerevisiae | (R)-S-lactoylglutathione | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | single polypeptide with two active sites that catalyze the same reaction | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
glyoxalase I | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1700 | - |
glutathione-methylglyoxal hemithioacetal | 30°C, pH 7.0 | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
S-hexylglutathione | - |
Saccharomyces cerevisiae |